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Analysis of monosaccharide residues composition in human erythropoietin
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Yi-fang AN, Xin-chang SHI, Lei YU, Xiang LI, Yong ZHOU
Chinese Journal of New Drugs | 2023, 32(8) : 853 - 857
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Chinese Journal of New Drugs | 2023, 32(8): 853-857
Analysis of monosaccharide residues composition in human erythropoietin
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Yi-fang AN, Xin-chang SHI, Lei YU, Xiang LI, Yong ZHOU
Affiliations
  • Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institute for Food and Drug Control, Beijing 100050, China
Published: 2023-04-30
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Objective:

To analyze the monosaccharide composition of human erythropoietin by cIEF-MS.

Methods:

The cIEF peak was obtained after cIEF detection, and each peak was detected by MS to obtain the high resolution mass spectral molecular weight. After deducting the protein molecular weight, the redundant molecular weight was compared with the molecular weight combination database of monosaccharide residues (accuracy being about 0.01%) to obtain a series of monosaccharide combination manners with basically consistent mass, and the rationality of monosaccharide quantity and the relationship between monosaccharides were analyzed.

Results:

Ten cIEF peaks were obtained by cIEF detection, and no less than 32 peak mass numbers were obtained by MS detection. After comparison, a series of monosaccharide combination forms corresponding to each mass number were obtained. Only the combination form with the highest ion number P3 was listed in this paper. The effectiveness analysis results showed that the most extensible combination manners were 19 GlcNAc/GlaNAc residues, 22 Man/Gla residues, 12 Neu5Ac residues and 3 Fuc residues.

Conclusion:

The monosaccharide combination manner of human erythropoietin protein glycosylation chain was obtained, which laid a foundation for the further analysis and characterization of complete protein glycoforms.

capillary isoelectric focusing-mass spectrum  /  human erythropoietin  /  isoelectric point isoform  /  N-glycosylation  /  O-glycosylation  /  monosaccharide residues composition
Yi-fang AN, Xin-chang SHI, Lei YU, Xiang LI, Yong ZHOU. Analysis of monosaccharide residues composition in human erythropoietin[J]. Chinese Journal of New Drugs, 2023 , 32 (8) : 853 -857 .
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  • Online Date：2026-03-06
  • Published：2023-04-30
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  • Accepted：2022-10-17
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    Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, National Institute for Food and Drug Control, Beijing 100050, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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