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Vitamin D is an essential fat-soluble vitamin, which plays a very important role in maintaining the dynamic balance of calcium in human body and can prevent calcium deficiency diseases such as osteoporosis and rickets. Both excess and deficiency of vitamin D pose a threat to human health, so it is necessary to establish methods for monitoring vitamin D concentrations in different matrices (e.g., pharmaceuticals, foods, biological samples, etc.). However, due to the unstable chemical properties of vitamin D and the complex matrix of vitamin D-containing samples, the pre-treatment and determination of vitamin D-containing samples have a great challenge. This paper reviews the research progress of pretreatment and determination of vitamin D in different matrices in recent years. It is reported that the most commonly used pretreatment methods are liquid-liquid extraction, solid-phase extraction and liquid-liquid microextraction, and the determination methods mainly include high performance liquid chromatography, mass spectrometry, supercritical fluid chromatography and other methods. This review is expected to provide technical support for the determination of vitamin D series preparations.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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