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To prepare the β-cyclodextrin inclusion complexes of volatile oil in Qizhu granules.
The technology parameters like inclusion time, inclusion temperature, and the ratio of volatile oil to β-cyclodextrin in the preparation process of inclusion complexes were investigated by single factor experiment with inclusion rate as the index. With inclusion rate and inclusion yield as indexes, Box-Behnken response surface method was used to optimize the preparation process of inclusion complexes. Thin-layer chromatography, infrared spectroscopy and differential scanning calorimetry were utilized to characterize the inclusion complexes.
The optimal conditions were as follows: 9∶1 for volatile oils-(β-cyclodextrin) ratio, 64 ℃ for inclusion temperature, and 1 h for inclusion time. The inclusion rate and yield of the obtained inclusion complexes were 90.68% and 80.40%, respectively. All characterization results revealed the formation of inclusion complexes.
The established method is stable and feasible, which can be used for preparing the β-cyclodextrin inclusion complexes of volatile oil in Qizhu granules．
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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