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To isolate and prepare iridin monomer from traditional Chinese medicine Iridis tectori Rhizoma, and to explore the regulatory effect of iridin on the isolated intestinal muscle in vitro.
The multi-solvent extraction method was used to prepare Iridin. The effect of iridin on acetylcholinesterase (AChE) activity was studied by modified Ellman colorimetry. The isolated intestinal muscle experiment was used for in vitro evaluation. In this study, the mouse small intestinal smooth muscle was obtained, from which the small intestinal smooth muscle ring was prepared in K-H solution. The change of the tension was measured by the BL-420S biological function experimental system. The resting tension and its effects on the contraction of small intestinal smooth muscle induced by neostigmine and pralidoxime iodide were investigated.
The iridin monomer obtained by the multi-solvent extraction method has a purity of over 98% calculated by the HPLC peak area normalization method. Iridin had inhibitory effect on AChE activity with a dose-dependent manner, and its half-inhibitory concentration (IC50) is 4.09 mg·mL-1. Iridin (0.014~4.604 mg·mL-1) had obvious excitatory effect on the contraction of isolated small intestine in normal mice, and the inhibitory effect was dose-dependent. Iridin had a significant antagonistic effect on the inhibition of small intestinal contraction caused by the cholinesterase revitalizer iodopyridine (P<0.05), but had no significant synergistic effect on promoting the small intestinal motility by the cholinesterase inhibitor neostigmine (P>0.05).
Multi-solvent extraction method is a simple and efficient method for the isolation and preparation of iridin in Iridis tectori Rhizoma, and iridin has the cholinergic effect on inhibiting AChE. It can promote the contraction of the small intestine, and is expected to be a candidate for ache inhibitor.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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