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The present study explored the active ingredients and potential mechanism of Wuwei Ganlu yaoyu decoction powder in the treatment of gouty arthritis(GA).
The main chemical components of Wuwei Ganlu yaoyu decoction powder were identified by UPLC-Q-Exactive-Orbitrap-MS. The target compounds were screened from the identified compounds by Swiss Target database. The databases genecards, OMIM and others were used to screen disease targets, STRING to build PPI network and select key targets, DAVID to enrich GO and KEGG pathways of common targets of drug diseases. Based on these, we discussed the mechanism of Wuwei Ganlu yaoyu decoction powder in the treatment of GA. In addition, the "drug-component-target-disease" network was constructed by using Cytoscape 3.9.1 software, and Maestro software was used to conduct molecular docking between components and core targets to screen effective components.
A total of 52 compounds were identified, including 24 flavonoids, 5 alkaloids, 10 organic acids, 5 phenols, 5 phenylpropanoids, 2 terpenoids and 1 alkane. According to the analysis of network pharmacology, there were 520 component targets and 141 intersection targets of Wuwei Ganlu yaoyu decoction powder, and 5 core targets were screened (SRC, STST3, PIK3CA, MAPK1, HSP90AA1). Molecular docking showed that six components such as Luteolin had good binding ability with five core targets.
The active components of Wuwei Ganlu yaoyu decoction powder in the treatment of GA are luteolin, hyperoxin-7-O-β-D-glucopyranoside, Hyprolatine-7-O-β-D-xylopyranoside, quercetin, naringin and luteolin, which may be related to the action on targets like SRC, STAT3, PIK3CA and others to regulate the signal pathways of PI3K Akt, Ras, MAPK, etc.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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