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To study the cytotoxicity effect of NK cells on breast cancer cells in vitro, and the cytotoxicity and possible mechanism of NK cells combined with low concentration lapatinib on breast cancer cells.
CCK-8 method was used to detect the effect of different concentrations of lapatinib on the proliferation of breast cancer cells for 24 h, 48 h and 72 h. LDH Assay Kit was applied to detect cytotoxicity of NK cells and NK cells combined with lapatinib on tumor cells. Changes of HER-2 expression on the surface of MDA-MB-231 and BT549 breast cancer cells before and after treatment with lapatinib and the ligand expression of NK cell activated and inhibitory receptors on breast cancer cell surface were both evaluated by flow cytometry.
The inhibitory effect of lapatinib on the proliferative activity of breast cancer cells was proportional to the time and concentration of action. The inhibitory rate on the proliferation of HER-2 high-expression breast cancer cells was greater than that of HER-2 low-expression breast cancer cells. NK cells had certain cytotoxicity against breast cancer cells, and lapatinib (The final concentration of lapatinib was 0.1 μmol·L-1 in HER-2 high-expression breast cancer cells MDA-MB-453 and BT474 and 3.125 μmol·L-1 in HER-2 low-expression breast cancer cells MDA-MB-231 and BT549) combined with NK cells could significantly improve the killing efficiency of NK cells against breast cancer cells. Lapatinib (3.125 μmol·L-1) could increase the expression level of HER-2 on the surface of HER-2 low-expression breast cancer cells after interacting with HER-2 low-expression breast cancer cells.
Lapatinib improves the killing activity of NK cells against breast cancer cells in vitro, and its synergistic mechanism may be related to the increased expression of HER-2 on the surface of breast cancer cells by lapatinib.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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