PDF
Full
Outline
To establish a detection method for exogenous DNA in neomycin sulfate drugs based on the concept of analytical quality by design (AQbD).
The MODDE Pro11 software was used to design the experiment, investigate the key influencing factors and optimize the parameters. Qubit® DNA detection kit based on fluorescence staining method was employed for the determination of exogenous DNA.
The universal detection method of exogenous DNA in neomycin sulfate was established. The key effect factors are solvent type, solvent concentration and sample concentration. When NaCl was used as solvent, the optimal analysis conditions were solvent concentration at 1.4 mol·L-1 and sample concentration at 1.8 mg·mL-1. The linear range of DNA standard was 20~100 ng·mL-1 (r=0.995 7, n=2). The accuracy was above 81.0%. The recoveries in ruggedness tests were 80%~120%.
The established method is accurate, robust and universal. This study provides reference for the development of determination of exogenous DNA in aminoglycoside antibiotics.
PDF
33
15
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
