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To establish the quality standard of Xianqian Granules.
According to the relevant methods presented in the fourth general rules of Pharmacopoeia of the People's Republic of China (2020), TLC method for identifying 4 Chinese medicinal materials in Xianqian Granules, including Agrimoniae Herba, Rubiae Radix et Rhizoma, Paeoniae Radix Rubra, and Notoginseng Radix et Rhizoma, was established, as well as the HPLC method for determining the index components of purpurin and paeoniflorin in Xianqian Granules.
The TLC method was specific to identify the 4 medicinal materials effectively. In the quantitative analysis method, the mass concentrations of purpurin and paeoniflorin were linear in the ranges of 2.47~53.00 μg·mL-1 and 18.29~392.0 μg·mL-1, respectively. The average recoveries were 97.37% (RSD 1.44%) and 99.04% (RSD 0.82%), respectively.
The established methods for quality evaluation of Xianqian Granules are simple, accurate, and specific, which can be used for the quality control for Xianqian Granules.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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