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To establish the HPLC-PDA fingerprint of Folium Photiniae combined with chemometrics to identify and screen the quality markers of Folium Photiniae, in order to provide a scientific basis for the quality of Folium Photiniae.
The fingerprint was obtained by an Agilent Eclipse XDB-C18 (250 mm×4.6 mm,5 μm) chromatographic column with a mobile phase of acetonitrile (A)-0.1% formic acid aqueous solution (B) in a gradient elution. The volume flow rate was set at 1.0 mL·min-1, the wavelength was 350 nm and the column temperature was 35 ℃. The fingerprints of 15 batches of Folium Photiniae were established using the developed method and the similarity was evaluated. The common peaks were analyzed by chemical identification pattern to distinguish Folium Photiniae from different origins, and the quality markers of Folium Photiniae were screened.
The similarity of the 15 batches of Folium Photiniae were all above 0.996, and a total of 10 common peaks were calibrated. Cluster analysis, principal component analysis and partial least squares analysis screened out chromatographic peak No.1 (neochlorogenic acid), No.2 (chlorogenic acid), No.4 (rutin), No.5 (hyricoside), and No.6 (isoquercitrin) as the quality markers of Folium Photiniae. The contents of neochlorogenic acid and chlorogenic acid is positively correlated with the quality of Folium Photiniae. The contents of rutin, hypericin and isoquercitrin may be negatively correlated with the quality of Folium Photiniae, indicating that the contents of these three components in Folium Photiniae should be limited at a low level. Further quantitative analysis of the above five components indicated the linear ranges of 0.135 3~2.706 0, 0.134 0~2.680 0, 0.008 5~0.170 5, 0.015 6~0.312 8, 0.005 9~0.118 9 μg, respectively. The linear relationship of each component is good (R2≥0.999 0), and the average recovery rate is 99.30% to 101.90%.
The established HPLC fingerprint and multi-component determination method are proved to be stable and reliable, which can provide a basis for the quality evaluation of Folium Photiniae.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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