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To investigate the anti-neuroinflammatory effect of Aucubin extracted from Rehmannia glutinosa in lipopolysaccharide (LPS)-induced neuroinflammation model in vitro.
N9 cells were activated by 10 μg·mL-1 LPS to establish an in vitro neuroinflammatory model. After treatment with Aucubin for 24 h, NO content was detected by Griess method, cell viability was detected by MTT method, cell morphology was photographed by microscopy, the level of Iba-1 (a specific marker of microglia) was detected by immunofluorescence, CD11b level and CD86/CD206 ratio were determined by flowsight assay, and the levels of IL-6, IL-1β, IL-4 and IL-10 were detected by kits.
Compared with the model group, the aucubin group effectively improved cell morphology, reduced cell supernatant NO level, decreased cell marker CD11b level and CD86/CD206 value, and regulated the release of inflammatory factors.
Aucubin inhibited the activation of microglia to M1 phenotype and promoted the transformation of M2 phenotype. It also regulated the release of inflammatory factors, thus inhibited the activation of N9 microglia and finally suppressed LPS-induced neuroinflammation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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