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To evaluate the pharmacokinetic characteristics of Jinzhen oral liquid and compare the difference of the absorption and metabolism after single and multiple administrations in rats.
An ultra-high performance liquid chromatography coupled to triple-stage quadrupole mass spectrometer (UPLC-TSQ-MS) was applied to established the method for simultaneous determination of baicalin and wogonoside in rat plasma. Healthy rats were administered a single dose (3.228, 6.456, and 12.912 g·kg-1) and multiple dose (6.456 g·kg-1) of Jinzhen oral liquid (crude drug) by oral gavage. The content of target components in plasma at different time points was determined, and the pharmacokinetic parameters were calculated using the non-compartmental model.
The two components in the plasma sample have good linear relation (r>0.99). RSDs of their extraction recovery rate and matrix effect are both <15%, the intra- and inter-batch precision and accuracy, and stability meet the requirements of biological sample analysis. After single administration of Jinzhen oral liquid, the areas under the concentration-time curve AUC(0-t) of baicalin and wogonoside show a good positive correlation with dose (R2>0.9), and their t1/2z and CLz/F have no significant difference at different doses. Compared with a single administration, their absorption and metabolism process are basically the same, and their AUC(0-t) and t1/2z have no significant difference after multiple administration.
A stable and reliable UPLC-TSQ-MS quantitative method for baicalin and wogonoside was established. And two components of Jinzhen oral liquid show linear dynamics characteristics in rats between 3.228 and 12.912 g·kg-1 (crude drug), and there is no accumulation after multiple administrations, revealing the rationality of the clinical dosage of the preparation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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