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To investigate whether β-caryophyllene (BCP) can reduce cerebral ischemia-reperfusion injury (CIR) in rats via activating heat shock factor 1 (HSF1)/heat shock protein 70 (HSP70) pathway.
Male SD rats were randomly assigned to the sham operation group, model group, BCP low, medium, and high dose groups (204,306,408 mg·kg-1), BCP (306 mg·kg-1)+Kribb11 intervention group, and Kribb11 intervention group. The suture method was used to create a rat model of CIR injury, and the neurobehavioral score was recorded 24 hours after the reperfusion. The volume of the cerebral infarct was measured using 2,3,5-triphenyltetrazolium chloride staining, and the pathological changes of cortical neurons were observed using hematoxylin-eosin staining and Nissl staining. Western blotting was applied to detect protein expressions of HSP70, HSF1, p-HSF1, Chop, Caspase 3, Bcl2, and Bax in rat brain cortex; immunofluorescence staining was used to detect protein expressions of p-HSF1 and HSP70; and Tunel staining to evaluate cell apoptosis level. Malondialdehyde level in cortical tissue (MDA), activities of total superoxide dismutase (SOD) and Caspase 3 were measured using a chemical colorimetric technique.
Compared to the model group, the β-caryophyllene administration group indicated a lower cerebral infarct volume in rats (P<0.01), a lower level of MDA and Caspase 3 activity in the tissue (P<0.01,P<0.05), a decreased expression of Bax, Chop, and Caspase 3 protein (P<0.01), a decreased occurrence of cell apoptosis, and increased protein expressions of HSF1, p-HSF1, HSP70, and Bcl 2 in the tissue (P<0.01), and substantially greater SOD activity in the tissue (P<0.01), reducing ischemia-reperfusion injury. Compared with the BCP group, Kribb11 in the BCP+Kribb11 group antagonized the curative effect of BCP, the protein expressions of HSF1, p-HSF1 and HSP70 were significantly reduced (P<0.05, P<0.01), and tissue oxidation stress levels increased, cortical cell death increased, and ischemia-reperfusion injury aggravated.
In rats, β-caryophyllene can alleviate cerebral ischemia-reperfusion injury in rats by reducing oxidative stress and minimizing cell apoptosis, which is associated with the activation of the HSF1/HSP70 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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