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Objective: To explore the effective compatibility ratio of the 5 main active ingredients in Gardenia and Panax notoginseng by uniform design test method thus to provide preliminary experimental evidence for clinical treatment of Alzheimer's disease. Methods: A model of SH-SY5Y cell injury induced by β-amyloid (Aβ1-42) was established, and the cell survival rate was used as an indicator to study the effective concentrations of the main active ingredients against Aβ1-42-induced SH-SY5Y neuronal injury, and according to the damage recovery rate, five ingredients with better efficacy were selected. On this basis, the five components with good efficacy were used as independent variables X1, X2, X3, X4 and X5, respectively, the injury recovery rate (%) as the dependent variable Y, U21 (215) uniform design table and 2 mathematical models of linear and quadratic polynomials were selected, and the variables were screened by stepwise regression method to determine the effective compatibility ratio of the 5 active ingredients. Results: The main active components of Gardenia-Panax notoginseng, except genipin, could increase the cell survival rate of SH-SY5Y cells induced by Aβ1-42 in a concentration-dependent manner. 5 ingredients with good efficacy were identified as notoginsenoside R1, ginsenoside Rb1, ginsenoside Rg1, geniposide, and genipin gentiobiglycoside. The results of uniform design experiments were obtained by regression analysis and Y=12.775 019 92-0.493 827 042 7X2+0.070 634 665 58X1X2-0.022 126 968 533X1X5+0.053 000 691 95X3X4 (r=0.857 4, F=11.105 9, P=0.002), and the uniform design test verified that the best combination ratio was notoginsenoside R1∶ginsenoside Rb1∶ginsenoside Rg1∶geniposide∶genipin gentiobiglycoside=1∶1∶1∶1∶0. Behavioral experiments showed that compared with the model group, the number of tests and memory errors of donepezil hydrochloride group and gardenia-Panax notoginseng optimal matching were significantly reduced. The results of ROS and Western blot showed that compared with the model group, the optimal combination of donepezil hydrochloride group and gardenia-Panax notoginseng could effectively reduce the fluorescence intensity of ROS and the expression of RAGE protein. Conclusion: The optimal matching ratio obtained by uniform design screening can effectively improve Alzheimer's disease, and its mechanism of action may play an anti-Alzheimer's effect by inhibiting the activation of RAGE-ROS pathway, which provides an experimental basis for the treatment of Alzheimer's disease.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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