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Objective: To study the stability of hepatocyte growth-promoting factor for injection (HGFI) and its compatibility with different material infusion sets by physical and chemical testing and cell experiment. Methods: HGFI was dissolved with different solvents. Then the solution was sampled at 0 h and 24 h with or without infusion tube made of polyvinylchloride (PVC), thermoplastic elastomer (TPE), of thermoplastic polyurethanes (TPU) to determine the pH value, protein, clarity, visible foreign bodies, insoluble particles, osmolarity, content and macromolecular substances. HGFI was dissolved with 5% glucose injection or normal saline for 0 h and 24 h, and then diluted with complete medium and co-incubated with rat liver cells (BRL) for 48 h. The cell viability was detected by CCK-8 method. Results: The pH value, protein, degree of clarification, visible foreign bodies, insoluble particles, osmotic molar concentration, content determination, and macromolecular substances of HGFI dissolved with 5% glucose, normal saline, 5% glucose and normal saline and sampled via/not via infusion tube of different materials (PVC, TPE, TPU) at 0 h and 24 h all met the requirements of 2020 edition of Chinese Pharmacopoeia. Compared with the normal control group, HGFI solutions at 0 h and 24 h not only had no toxic effects on BRL cells, but also significantly increased the viability of BRL cells. Conclusion: HGFI is stable within 24 h with different solvents and has good compatibility with infusion sets of different materials. Moreover, it keeps wonderful bioactivity within 24 h.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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