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To determine the index weight coefficient, optimize the ultrasonic extraction process of flavonoids in Scutellaria baicalensis Georgi and analyze its anti-inflammatory activity in vitro.
On the basis of single factor test, ethanol concentration (A), ultrasonic time (B), ultrasonic temperature (C) and material-to-liquid ratio (D) were selected as the investigation factors. Taking baicalin, wogonin, baicalein and wogonin as the inspection indicators, the AHP-CRITIC mixed weighting method was used to determine the weight coefficients of each index, multivariate binomial was fitted combined with the response surface method, a three-dimensional graph was drawn, and the best extraction process was chosen. At the same time, the MTT method and Griess method were used to evaluate the in vitro anti-inflammatory activity of the ultrasonic extraction of Scutellaria baicalensis Georgi.
The optimal extraction conditions for flavonoids in Scutellaria baicalensis Georgi were as follows: ethanol concentration of 62.73%, ultrasonic time of 32.23 min, ultrasonic temperature of 51.15 ℃, and material-to-liquid ratio of 1∶16.12. The average AHP-CRITIC comprehensive score of three batches of verification samples was 85.28, and the RSD with the theoretical prediction value was 0.50%. The results of MTT method showed that when the concentration of Scutellaria baicalensis Georgi extraction was greater than 400 μg·mL-1, the cell viability decreased significantly (P<0.05, P<0.01). When the concentration of Scutellaria baicalensis Georgi extraction was 50~350 μg·mL-1, the release of NO in RAW264.7 inflammatory cells was significantly inhibited (P<0.05, P<0.01).
The method is stable and reliable and can be used for the extraction of flavonoids from Scutellaria baicalensis Georgi, and the ultrasonic extract has strong anti-inflammatory activity in vitro.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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