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To prepare photothermal lipid membrane coated Escherichia coli and study the properties.
The photothermal lipid membrane (LMI) was prepared by thin film dispersion method, and the electrostatic adsorption method was used to coat Escherichia coli with the lipid membrane (LMI@Ec). The size and potential of LMI@Ec were analyzed by laser scattering particle size analyzer, the morphology of E.coli and LMI@Ec was observed by transmission electron microscopy and laser confocal microscopy, and plate counting method was used to detect cell viability of LMI@Ec. In addition, the effect of protein expression of LMI@Ec was evaluated by polyacrylamide gel electrophoresis, and the photothermal effect of LMI@Ec was detected by Infrared Photothermal Imager.
LMI@Ec had a smooth surface with the average particle size of (1 990.5±132) nm and the potential of -(5.09±0.52) mV. The coating of the lipid membrane had little effect on the activity of E.coli. Although the bacterial activity decreased after laser irradiation, the growth curve recovered to the same level as wild bacteria after 10 h of temperature gradient culture in LB medium. LMI@Ec had well photothermal properties and can normally express protein. In vitro cytotoxicity experiments showed that LMI@Ec under 808 nm laser irradiation presented enhanced antitumor effect on B16F10 and HepG2 cells.
The successful preparation of LMI@Ec provides a new research direction for the subsequent in-depth study of bacteria-mediated disease treatment.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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