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To explore the effect and mechanism of compound cortex phellodendri fluid in bacterial vaginosis rats via TLR4/NF-κB pathway.
Fifty-five SD female rats were selected to construct bacterial vaginosis model by injection of mixed bacterial liquid. After successful modeling, the rats were treated with compound cortex phellodendri fluid, metronidazole vaginal gel, and Honghe fujie lotion, respectively. After 7 d of continuous treatment, inflammatory cell infiltration and glycogen accumulation in rat vaginal tissues were observed by HE staining. ELISA method was used to detect the expression level of IL-1β, IL-2, IL-10, IL-6, and TNF-α in the vaginal lavage. The distribution of NF-κB p65 in rat vaginal tissues were located by immunohistochemical method. And the protein expression level of TLR4 and NF-κB p65 were detected by Western blot.
Compared with those of the model group, the infiltration of inflammatory cells in compound cortex phellodendri fluid group was reduced, and the accumulation of glycogen was significantly increased (P<0.01), the concentrations of IL-1β (P<0.01), IL-6 (P<0.05), IL-2, and TNF-α in the vaginal lavage were decreased, while the IL-10 concentration increased (P>0.05), the protein expression level of TLR4 and NF-κB p65 decreased (P<0.05), and the NF-κB p65's translocated nuclear were suppressed (P<0.01).
Compound cortex phellodendri fluid has a definite therapeutic effect on bacterial vaginosis rats. Its mechanism may be related to the inhibition of TLR4/NF-κB pathway and adjustion of immune imbalance on Th1/Th2.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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