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To study the distribution of expanded activated lymphocytes in severe immunodeficient mice.
Ninety-six NPG mice were used and randomly divided into vehicle control group and administration group. The animals in the vehicle control group were given vehicle by tail vein injection once; the animals in the administration group were given DiR-labeled EAL cells by tail vein injection once. Blood was collected at different time points after administration, and EAL cells were detected by in vivo imaging system. At 1 h, 3 h, 2 d, 7 d, 14 d, 28 d, 42 d, and 56 d after administration, the mice were anesthetized, and blood, heart, liver, spleen, lung, kidney, brain, testis, epididymis, uterus, ovary, stomach, duodenum, colon, bone marrow, fat, and skeletal muscle were taken for cryopreservation. The distribution of EAL cells in peripheral blood and the above-mentioned tissues was studied by flow cytometry, in vivo imaging method and qPCR method.
The numbers of CD3+ T cells, CD3+CD4+ T cells and CD3+CD8+ T cells in peripheral blood were higher at 2 d after administration, and then showed a downward trend, reaching the lowest at 14 d, and maintained a low level thereafter. The results of in vivo imaging showed that the cells were mainly distributed in the lungs, liver, spleen and bilateral leg bones, and were mainly distributed in the lungs and liver within 1 d after administration. The results of gene copy number detection showed that the cells were mainly distributed in the lungs and blood, followed by the spleen, liver and bone marrow, and only a little in other tissues. At 1 h after administration, the gene copy number of cells in the lungs was relatively high. At 2 d, the number of gene copies in the blood and spleen was relatively high, and then gradually decreased, reaching the lowest at 14 d, and at 56 d cells were only detected in individual organs of one mice.
EAL cells were administered to NPG mice, the cells were mainly distributed in lung, blood and spleen. At 56d after administration, the cells were basically eliminated from the mice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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