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To establish the method of simultaneous determination of 12 major chemical components (such as berberine, magnolflorine and curcumin) in Tibetan medicine Ji-Ni De-Xie.
The separation was achieved on a WondaSil C18-WR column. High performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-QqQ-MS) was used with the mobile phase 0.1% formic acid solution (A)-methanol (B) under gradient elution. The temperature of Electron Spray Ionization (ESI) was 300 ℃. The charged ions were scanned in positive and negative ion modes at the same time with multiple reaction monitoring. The contents of 12 components in 5 batches of samples were calculated according to the linear equation of 12 reference substances.
The 12 components of the Ji-Ni De-Xie have a good linear relationship within the detection range. The average contents of magnolflorine, jatrorrhizine, berberine, curcumin, demethoxycurcumin, gallic acid, chebulagic acid, ferulic acid 4-O-β-D-glucopyranoside, hydroxysafflor yellow A, rutin, ferulic acid, and ellagic acid are 3.18, 0.20, 1.71, 0.52, 0.22, 15.93, 7.56, 3.29, 0.07, 0.20, 1.47, 2.89 mg·g-1, respectively.
The established analytical method is highly selective with high sensitivity. providing reference for the quality control of the Tibetan medicine Ji-Ni De-Xie.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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