Full
Outline
To establish an ultra-performance liquid chromatography-tandem triple quadrupole mass spectrometry (UPLC-TQ-MS/MS) method for simultaneous quantification of 10 components in rat plasma samples and investigate the pharmacokinetic characteristics of the main components after oral administration of Sanjie Zhentong Capsules (SZC).
Healthy SD rats were administered the contents of SZC at single doses of 1.73, 2.59, and 3.89 g·kg－1 by oral gavage. Plasma concentrations of 10 components were measured at different time points and pharmacokinetic parameters were analyzed by using non-compartmental model.
After systematic method validation, the 10 components exhibited good linearity (r>0.99). The intra-batch and inter-batch accuracy ranged from 85.3% to 113.8% and 91.6% to 109.9%, respectively. The relative standard deviations (RSDs) of extraction recovery and matrix effects were both below 15%. These results met the requirements of biological sample analysis, demonstrating that the method is specific, sensitive, accurate, and stable, thus suitable for quantifying the target components in plasma. Pharmacokinetic analysis revealed that the plasma exposure of loureirin D and pterostilbene was higher than that of other components, and five components (loureirin C, loureirin D, 7,4′-dihydroxyflavone, resveratrol, and pterostilbene) exhibited strong positive correlations (R2>0.91) between dosage and both AUC(0-t) and Cmax. Except for ginsenoside Rd, which exhibited prolonged retention in vivo, the other nine components demonstrated rapid absorption and fast elimination.
This study established a quantitative analysis method for 10 components of SZC in rat plasma. Within the dose range of 1.73~3.89 g·kg－1, five components of SZC exhibited linear pharmacokinetic characteristics in rats. These findings further clarify the pharmacodynamic material basis of this preparation and support its clinical use, providing data to improve the quality standards.
47
9
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
