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Outline
To systematically investigate the dynamic tissue distribution and tumor-targeting characteristics of memory T cells in non-tumor-bearing and tumor-bearing NCG mouse models using validated qPCR and in vivo imaging techniques.
A total of 144 NCG mice were randomly divided into non-tumor-bearing control, tumor-bearing control, non-tumor-bearing treatment, and tumor-bearing treatment groups. The treatment groups received a single intravenous injection of 1×107 memory T cells per mouse, while the control groups received vehicle only. In vivo imaging was performed on days 1, 3, 7, 14, 21, and 28 post-administration. Tissues and body fluids were collected at the same time points, and the genomic distribution of memory T cells was quantified by qPCR.
In non-tumor-bearing mice, memory T cells were initially (day 1~3) distributed mainly in highly perfused organs such as the lung, spleen, and liver, followed by a rapid decline and near clearance by day 14. In tumor-bearing mice, the early distribution pattern resembled that in non-tumor-bearing animals; however, starting from day 14 onward, significant tumor enrichment was observed and persisted until day 28, accompanied by a concurrent decrease in cell concentrations in non-target tissues.
Memory T cells demonstrate a trend of rapid clearance in the absence of antigenic stimulation, whereas in the presence of tumor, they exhibit antigen-driven tumor homing and long-term persistence capabilities.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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