Full
Outline
To evaluate the in vivo dynamic change patterns and bioavailability of 5 main components in Yinqiao Qingre tablets in rat plasma.
A UPLC-TQ-MS method was established and validated for the simultaneous quantification of the 5 components in plasma. Rats were randomly divided into three groups: Group 1 was given Yinqiao Qingre tablets by intragastric administration (ig) at a dose of 0.153 7 g·kg-1 crude drug; Group 2 was given a mixed solution of the 5 components at the same concentration as that in the first group via ig; gGroup 3 was intravenously injected (via tail vein) with the mixed solution of the 5 components at a dose of 1 mg·kg-1. Plasma concentrations of the 5 components at different time points were determined. Pharmacokinetic parameters were calculated using a non-compartmental model, and absolute and relative bioavailability values were further computed.
The specificity of the 5 components analysis methods in plasma was good, the linear relationship was good (r>0.99), the extraction recovery and matrix effect RSD were less than 15%, and the precision, accuracy and stability within and between batches met the requirements. The average peak time (Tmax) of the five components was 0.083~1.35 h, and the terminal elimination half-life (t1/2) was less than 4 h. Moreover, the compound preparation could prolong the elimination half-life and in vivo residence time of the components. The absolute bioavailabilities of chlorogenic acid, puerarin, isoferulic acid, caffeic acid, and ferulic acid were 0.84%, 1.50%, 45.17%, 23.82%, and 32.97%, respectively, while their relative bioavailabilities were all relatively high (ranging from 82% to 94%).
The established analytical method for plasma samples is rapid, stable, and accurate. Yinqiao Qingre tablets can be rapidly absorbed into the blood after oral administration, with isoferulic acid showing the highest plasma exposure and absolute bioavailability. The compatibility of the compound preparation is beneficial for the full exertion of therapeutic effects, and has little impact on the absolute bioavailability of the 5 components.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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