Full
Outline
To establish an HPLC-PDA method for the simultaneous determination of 5-hydroxymethylfurfural, chlorogenic acid, caffeic acid, isochlorogenic acid A, cichoric acid, baicalin, oroxylin A-7-O-β-D-glucuronide, wogonoside, baicalein and glycyrrhizic acid in the Puqin Jiedu Lishi Granules.
The analysis was performed on a Waters Symmetry C18 column (250 mm×4.6 mm, 5 μm) with gradient elution using acetonitrile and 0.2% phosphoric acid solution as the mobile phase. The flow rate was 1.0 mL·min-1, the column temperature was 20 ℃, and the detection wavelengths were set at 284 nm, 327 nm, 280 nm and 254 nm.
The linear ranges of 5-hydroxymethylfurfural, chlorogenic acid, caffeic acid, isochlorogenic acid A, cichoric acid, baicalin, oroxylin A-7-O-β-D-glucuronide, wogonoside, baicalein and glycyrrhizic acid were 1.860~59.64, 5.425~173.6, 1.980~63.36, 1.890~60.24, 5.990~191.6, 33.30~1066, 6.440~206.0, 28.00~896.0, 2.044~65.40, 6.660~213.1 μg·mL-1, respectively, with the correlation coefficients r≥0.999 5. The precision, stability, and reproducibility tests yielded satisfactory results. The average recoveries of the 10 components ranged from 98.15%~102.21%, with RSDs between 0.96%~1.91%.
This method is reliable, stable and reproducible, providing a reference for the quality control of Puqin Jiedu Lishi Granules.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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