Objective: A high-fat diet is a risk factor for cognitive impairment， but its underlying biological mechanisms have not been elucidated. Methods: twenty-four male 3-month-old C57BL/6J mice were randomly divided into two groups， and fed with normal chow diet (ND) and high-fat diet (HFD) for 12 weeks. At the end of the experiment， the glucose tolerance test (GTT) was performed. Cognitive function was evaluated with the Morris Water Maze (MWM) test. After overnight fast， the mice were sacrificed and serum and colon contents were collected. Serum insulin levels were detected by ELISA， serum glucose levels were determined by colorimetric method and HOMA-IR was calculated. Liquid chromatography tandem mass spectrometry was used to detect serum BCAA levels. 16S rDNA amplicon sequencing was used to detect gut microbiota alterations， and their correlation with serum BCAAs was performed by Spearman correlation. Results: After 12 weeks feeding， compared with the control mice， the HFD-fed mice showed significant increases in body weight (31.6 g vs 37.5 g， P<0.001)， body fat rate (3.2% vs 5.8%， P<0.001)， area under curve (AUC) of GTT (1 440 mmol/L·min vs 1 841 mmol/L·min， P<0.05)， and fasting serum levels of isoleucine (1.27-fold of the control group， P<0.05) and total BCAAs (1.16-fold of the control group， P<0.05). Additionally， the fluorescence intensity of NeuN-positive immunoreactive regions in the dentate gyrus of the hippocampus was significantly decreased in the HFD group (27 vs 12， P<0.01). HFD-fed mice displayed a decrease in the number of platform and the target quadrant entries during the probe trial compared with the control group， and these indicators were negatively correlated with serum isoleucine and total BCAA levels. In addition， the composition of gut microbiota in HFD group was significantly different from that in the control group. At the genus level， Romboutsia and Blautia are the dominant microbiota in the colon of HFD-fed mice. Bacterial functionality prediction indicated that gut microbiota genes involved in BCAA degradation were significantly reduced in HFD group compared with the control group. The Parvibacter genus is negatively correlated with isoleucine levels (P=0.01)， while the Blautia and Anaerotruncuus genus are positively correlated with isoleucine levels (the P-value were 0.00 and 0.03， respectively). Conclusion: Gut microbiota may be the link between elevated serum BCAA levels and HFD-induced cognitive impairment.

The effects of high-altitude hypoxic environments on the human body are a hot topic in biomedical research， particularly regarding their impact on bone health， which is increasingly gaining attention. This study simulated a high-altitude hypoxic environment at 6 000 meters to establish a hypoxia mouse model， aiming to investigate the influence of bone peptide on the changes in bone structure in mice exposed to this environment. C57BL/6N mice aged 6 to 8 weeks were randomly divided into a blank control group， a model group， a Rhodiola oral solution group， and low， medium， and high-dose bone peptide groups， with 8 mice in each group. The blank control group was raised in a normoxic environment and received deionized water via gavage， while the other groups were placed in a low-pressure oxygen chamber simulating the high-altitude hypoxic environment. The model group received deionized water via gavage， while the positive drug group and the bone peptide low， medium， and high-dose groups were administered Rhodiola oral solution and bone peptide solutions respectively， for a treatment duration of 14 days. After the final administration， serum， organs， and femurs were collected from the mice. The levels of bone gamma-carboxyglutamate protein (BGP) and alkaline phosphatase (ALP) in the serum were measured， and the Micro-CT scans were used to assess changes in bone structure in the femurs of the mice. The results showed that， compared with the blank control group in normoxic conditions， the food intake and body weight of mice in the hypoxic environment decreased， with a significant drop observed in the first three days， followed by a gradual recovery. Compared to the model group， all doses of bone peptide significantly increased the serum levels of BGP and decreased the levels of ALP. The group receiving the high dose of bone peptide (2 000 mg/kg/d) showed the best results， with BGP levels increasing by 477.18% and ALP levels decreasing by 24.97%. Furthermore， the bone tissues of mice in the different bone peptide dosage groups showed increased contents of bone mineral， bone volume fraction， trabecular thickness， and trabecular number， while the trabecular separation decreased. These findings indicate that bone peptide can counteract the bone structural damage induced by high-altitude hypoxic environments and help prevent osteoporosis by increasing BGP levels， reducing ALP levels， and decreasing trabecular separation， thus maintaining the spatial structure of trabecular bone.

Sphingomyelin (SM) is one of the most abundant polar lipids in breast milk. However， the effects of SM intake in the early stage of life on the short-term and long-term health of infants and young children are unclear. This article takes Caenorhabditis elegans (C. elegans) as a model organism to explore the effects of sphingomyelin intervention in early life on short-term and long-term antioxidant and anti-aging effects. By analyzing the antioxidant stress capacity， antioxidant enzyme activity， glutathione content， malondialdehyde content， lipofuscin content， ROS content， antioxidant and anti-aging related gene expression during the L4 and adult stages of C. elegans， the mechanism of SM action was elucidated. Reproductive experiments have shown that supplementing milk source SM at concentrations of 0.2 μg/mL (LG) and 20 μg/mL (HG) during infancy is safe. Compared with the CG group， the average and maximum lifespans of the HG group were significantly prolonged， significantly prolonged by 3.75 days and 5.67 days respectively， consistent with changes in ROS levels in larvae and adults， and the stress resistance ability of both larvae and adults was improved. In terms of antioxidant enzymes， the activity of SOD enzyme activity (P＜0.001) and CAT enzyme activity (P＜0.05) and the GSH content (P＜0.01) in HG group larvae were significantly increased， and milk derived SM could significantly reduce the content of MDA in larvae. In the long-term development of life， the content of lipofuscin in HG group decreased (P＜0.001)， and after intervention， the activity of SOD enzyme activity (P＜0.001) and CAT enzyme activity (P＜0.001) was also significantly increased， and after intervention， both GSH content (P＜0.001) and MDA level (P＜0.001) were increased and decreased. In addition， supplementing with milk derived SM during infancy can upregulate the sod-3 and nsy-1 genes in larvae， while adults enhance their antioxidant and anti-aging abilities by upregulating the relative expression of sod-3， ctl-1， and daf-16 genes. In summary， supplementing with a certain amount of bovine milk derived SM during childhood can simultaneously enhance antioxidant and anti-aging abilities in both the short and long term of life.

In order to explore the suitable drying technology of fresh Potentilla anserina， six different drying technologies [hot air drying (HAD)， heat pump drying (HPD)， carbon fiber far-infrared drying (CFFD)， carbon fiber far-infrared combined heat pump drying (CFFCHPD)， vacuum pulse drying (VPD) and vacuum freeze drying (VFD)] were used to dry fresh Potentilla anserina. The effects of different drying technologies on the drying time， drying energy consumption， appearance quality (color， shape)， taste (physical property)， taste， rehydration ratio and other sensory characteristics， nutritional quality (reducing sugar， vitamin C) content of fresh Potentilla anserina were determined. The drying technology of fresh Potentilla anserina was comprehensively evaluated by analytic hierarchy process. The results showed that CFFCHPD could effectively shorten the drying time， which was 9.76%， 21.95%， 31.71%， 60.98% and 243.91% shorter than CFFD， HAD， HPD， VPD and VFD， respectively. The drying energy consumption of CFFCHPD， CFFD， HAD and HPD was significantly different (P<0.05). The drying time and energy consumption of CFFCHPD were the lowest， and VPD was the highest. After drying， the brightness of fresh Potentilla anserina became darker， and the color was reddish and yellowish. Except for VFD， the L* value of Potentilla anserina dried by other methods was significantly lower than that before drying (P<0.05)， and the a* value and b* value were significantly higher than those before drying (P<0.05). There was no significant difference in elasticity between different drying methods (P>0.05)， but there were significant differences in hardness， adhesiveness and chewiness (P<0.05)， among which HPD was the highest and VFD was the lowest. After drying， the richness of Potentilla anserina was significantly different； compared with VFD， the rehydration ratio of other drying methods decreased by 9.97%， 11.58%， 12.61%， 15.95% and 19.18%， respectively. After drying， the reducing sugar content of Potentilla anserine L. was significantly increased (P<0.05)， which was 111.21%， 106.57%， 97.92%， 93.46%， 82.96%， 60.28% higher than that of fresh Potentilla anserine L. respectively. Except for VFD method， the content of vitamin C was significantly decreased (P<0.05). The content of reducing sugar in VPD method was the highest and the content of vitamin C was the lowest. The content of reducing sugar in VFD method was the lowest and the content of vitamin C was the highest. The comprehensive evaluation results using the analytic hierarchy process were: CFFCHPD>HPD>CFFD>HAD>VFD>VPD， and the comprehensive score of CFFCHPD was the highest， followed by HPD. This study provides a reference for the application of modern drying technology of fresh jute.

To investigate the effects of drying methods on the structure characteristics and immunomodulatory activity of polysaccharides derived from Morchella esculenta， fresh M. esculenta were separately subjected to hot-air drying (HD) and freeze drying (FD). Polysaccharides from M. esculenta (MEP) extracted from the two dried samples were purified by macroporous adsorption resin column chromatography， yielding HD-treated MEP (HMEP) and FD-treated MEP (FMEP). The monosaccharides of HMEP and FMEP were consisted of mannose， glucose and galactose while with different molar ratios of 1∶0.34∶0.33 and 1∶4.50∶0.02， respectively. HD treatment caused the break of C—O—C glycosidic linkages in polysaccharides chains and enhanced the abundance of α-configuration in polysaccharides from M. esculenta. Both HMEP and FMEP improved immunity by inducing phagocytosis and the secretions of NO， TNF-α， IL-6 and IL-1β of RAW 264.7 cells. At 50 μg/mL， FMEP stimulated RAW 264.7 cells to secrete NO at a level of (35.29±1.25) μmol/L， which was higher than that induced by HMEP [(28.05±2.52) μmol/L] and the model group [(32.47±1.30) μmol/L] at 50 μg/mL. At 25 μg/mL， FMEP stimulated macrophage TNF-α secretion to (289.96±49.00) ng/mL， which was higher than that of HMEP [(188.56±13.60) ng/mL] and the model group [(97.76±2.00) ng/mL]. This indicates that FMEP exhibited higher immunomodulatory activity than HMEP. After comprehensive consideration， freeze drying would be recommended as a suitable drying process for use in the preparation of immunomodulatory polysaccharides from M. esculenta as functional foods.

To optimize the formulation of crab meatballs and investigate the quality maintenance technology during the freeze-thaw process， a response surface methodology was employed， utilizing the Box-Behnken experimental design following a single factor experiment. This approach significantly enhanced both the production technology and formulation of crab meatballs. The study examined the effects of varying concentrations of sodium alginate (SA) ice glaze solutions on several quality parameters of the crab meatballs， including ice glaze rate， transmittance， water vapor permeability， and water solubility. Additionally， the impact of freeze-thaw cycles on crab meatballs was assessed under three conditions: no coating， deionized water ice glaze， and 0.020 g/mL SA ice glaze， focusing on quality changes. The findings indicate that the optimal process conditions for crab meatballs involve the incorporation of ginger juice， crab meal， and salt at concentrations of 4.9%， 2.4%， and 2.0%， respectively. Under these conditions， the crab meatballs exhibited a hardness of 22.89 N， a sensory score of 37.56， and a cooking loss rate of 5.67%. The resulting meatballs were characterized by their elasticity， plump shape， and appealing color. Moreover， the 0.020 g/mL SA ice glaze solution was found to be the most effective for maintaining the quality of crab meatballs. After undergoing seven freeze-thaw cycles， the thawing loss and volatile base nitrogen values for the 0.020 g/mL SA ice glaze sample decreased by 25.54% and 35.67%， respectively， compared to the control. Conversely， chewiness and elasticity increased by 47.37% and 42.86%， respectively. Therefore， the 0.020 g/mL SA ice glaze significantly inhibits ice crystal formation， reduces water loss， and mitigates damage to meat texture， thereby enhancing the overall quality of the meat products.

To investigate the changes of textural properties and water characteristics of high-moisture extruded protein after cooking， soy protein concentrate was used as the raw material for high-moisture extrusion and the impact of three common cooking methods (steaming， boiling， and frying) and cooking time on the fibrous structure of the extrudates were investigated. The differences in cooking loss and shrinkage were evaluated， and changes in the macro- and micro-structure， textural properties and water characteristics of the extrudates were explored. Results showed that steaming preserved a better fibrous structure with moderate hardness and shear force， boiling loosened the fibrous structure and reduced hardness， chewiness and shear force of extrudates. Boiling for 20 min increased the moisture content of the extrudates by 10.38%. Both steaming and boiling increased T22 and T23， while reducing the proportion of bound water， suggesting a weakening of the protein-water binding in the extrudates. Frying enlarged the pores in the extrudates and enhanced the permeability of extrudates. Frying for 4 min increased the fat content of the extrudates by 3.65%， decreased the moisture content by 20.49% and reduced T21， T22， and T23 by 0.14， 7.31， 81.04 ms， respectively， while significantly increasing the proportions of bound water and free water (P<0.05). It can be concluded that different cooking methods could affect their fibrous structure， pore morphology and components， as well as the textural properties and water characteristics of the extrudates. This study provides a theoretical reference for the optimization of subsequent cooking process for high-moisture extruded plant-based meat products， with practical value in controlling their textural and water characteristics.

This study aimed to investigate the effects of different extraction temperatures on the chemical composition and flavor characteristics of coffee brews， in order to optimize atmospheric-pressure extraction and improve coffee flavor quality. Extraction temperatures (55-95 ℃) were adjusted to analyze the volatile and non-volatile compounds and sensory characteristics of coffees with different roast degrees (light， medium， and dark)， and to examine how temperature influences their chemical composition and flavor formation. Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were used to quantitatively analyze chemical components in coffee brews， and descriptive sensory evaluation was conducted to systematically assess sensory attributes. The results showed that extraction temperature significantly affected both the release of volatile compounds and the dissolution of non-volatile components (P<0.05). High-temperature extraction (95 ℃ and 85 ℃) increased extraction yield (>22%) and bitterness/astringency， whereas acidity and caramel notes were more pronounced at lower temperatures. Combined sensory and quantitative analyses indicated that high-temperature extraction improved extraction efficiency but led to the loss of some aroma compounds， while low-temperature extraction preserved more key aroma components. Significant differences in chemical composition and sensory properties were observed among coffees of different roast degrees at different extraction temperatures； higher temperatures were more suitable for comprehensive extraction of dark-roasted coffee， whereas lower temperatures were advantageous for aroma retention in light and medium roasts. This study provides theoretical support for optimizing coffee extraction processes， and future work will integrate other variables (e.g.， pressure and extraction time) for multidimensional research to further elucidate the mechanisms and control strategies of coffee flavor formation.

Objectives: This study aimed to optimize the extraction process of Zanthoxylum bungeanum seed oil to enhance the comprehensive utilization value of its by-products. Methods: The fatty acid composition of Zanthoxylum bungeanum seed oil was analyzed using gas chromatography-mass spectrometry. With the oil yield and the relative content of the main fatty acids as evaluation indices， the optimal extraction conditions were determined through single-factor experiments and orthogonal array design. Results: The main fatty acid in the oil was α-linolenic acid (relative content 23.39%). The optimal extraction conditions were as follows: Extraction temperature 60 ℃， extraction time 50 min， solid to liquid ratio 1∶3， ethanol volume fraction 90%， and extraction repeated three times. Under these conditions， the α-linolenic acid content reached 23.32%. Conclusions: This study clarifies the fatty acid profile of Zanthoxylum bungeanum seed oil and provides an important basis for its efficient extraction and for the resource utilization of Zanthoxylum bungeanum by-products.

Streptococcus thermophilus CCFM1095 in combination with Lactobacillus helveticus CCFM1263 significantly improves fermentation efficiency， but the mechanism of interaction has not been cleared. Therefore， this paper evaluates the fermentation characteristics of the complex strains and the metabolomic profiles of the fermented milks， and analyses the effects of key metabolites on fermentation characteristics. Metabolomics analyses showed that compounding significantly promoted the enrichment of pathways such as alanine， aspartate and glutamate metabolic pathways， lysine degradation pathway， TCA cycle， pyruvate metabolism and nicotinic acid and nicotinamide metabolic pathways， and increased the utilisation of citrate and nicotinamide by the strains Production of L-glutamate， fenugreek acid， α-ketoglutarate， and 4-trimethylaminobutyric acid lipids， which significantly promoted the growth of both strains. The fermentation characteristics of the complex fermented milk were significantly improved. The viable bacterial count of the strains could reach 108 CFU/mL. This study provides theoretical basis for further analysis of the interaction mechanism between Streptococcus thermophilus and Lactobacillus helveticus.