Objective: To study the expression levels of differentially expressed genes in Candida parapsilosis under high glucose stress and to investigate its stress response mechanism. Methods: The yeast presented in the contaminated condensed milk during storage were isolated and characterized by plate screening and 18s DNA sequencing， and the total RNA of the strains was extracted to construct cDNA libraries. Differentially expressed genes were analyzed by transcriptome analysis and verified by real-time fluorescence quantitative PCR. Results: The yeast was identified as Candida parapsilosis， its optimal temperature and pH value were 35 ℃ and 8， respectively. It could still grow slowly when the glucose concentration reached 50%. The main product in the fermentation broth was sorbitol， with a yield of up to 41.06 g/L. A total of 2 066 significant differential genes were detected in the transcriptomic analysis， of which 993 were significantly up-regulated and 1 073 were significantly down-regulated. The genes related to heat stress proteins (Hsp70， Hsp90)， amino acid transport， glycogen transport， glycolysis and ethanol dehydrogenase were significantly down-regulated， while the genes related to amino acid permease， glycogen synthesis， and synthesis of certain amino acids (e.g.， glutamate， proline， arginine， cysteine， etc.) with osmotic stress-protecting effects were significantly up-regulated. Conclusion: Candida parapsilosis could improve performance under hyperosmotic stress by synthesizing certain sugars or amino acids (e.g.， proline， glycine， and glutamate).

sweetened condensed milk  /  Candida parapsilosis  /  isolation and identification  /  transcriptomic analysis