[Objective] To understand the epidemic status of Infectious Bovine Rhinotracheitis （IBR） in large-scale dairy cattle farms in Shanghai from 2020 to 2024,and to provide a scientific basis for the prevention and control of IBR in this region. [Method] From 2020 to 2024,2 520 bovine serum samples were collected from cattle not vaccinated against IBR in 5 large-scale dairy farms located in 5 districts of Shanghai. Enzyme-linked immunosorbent assay （ELISA） was used to detect IBR gE antibodies and IBR gB antibodies in the serum. SPSS 27.0 software was used for statistical analysis of the test results,and the Chi-square test was applied to analyze the significance of differences in IBR virus antibody detection results among large-scale dairy farms in different districts. [Results] From 2020 to 2024,the overall positive rate for IBR gE antibodies in the tested serum samples was 25.7%,and the overall positive rate for IBR gB antibodies was 27.3%. Over the five years,the overall positive rates for both IBR gE and IBR gB antibodies showed a fluctuating upward trend. There were highly significant differences （P<0.01） in the positive rates of IBR antibodies among dairy farms in different districts. Chongming District had the highest positive rates for both IBR gE antibodies （57.2%）and IBR gB antibodies（74.4%）. Among cattle of different ages,adult cattle had the highest positive rates for IBR gE antibodies （36.2%） and IBR gB antibodies （32.9%）,while calves had the lowest positive rates for IBR gE antibodies（14.1%） and IBR gB antibodies（16.0%）. [Conclusion] From 2020 to 2024,IBR was prevalent to varying degrees in large-scale dairy cattle farms in Shanghai. Monitoring and control measures for IBR should be further strengthened,especially in high-infection-rate areas such as Chongming District and Jinshan District,where differentiated prevention and control strategies need to be adopted to effectively control the transmission and prevalence of IBR.

large-scale dairy cattle farm  /  Infectious Bovine Rhinotracheitis（IBR）  /  antibody  /  serological survey  /  prevention and control strategy