PDF
Full
Outline
To investigate the composition of psychrotrophic bacterial communities and the diversity of psychrotrophic microorganisms in dairy raw materials from different pastures and at different production times.
36 raw milk samples were collected from large-scale pastures in North China. To investigate the alterations in bacterial community composition and the variety of psychrotrophic bacteria in raw milk from various pastures and seasons, researchers employed both 16SrRNA sequencing and conventional isolation and culture techniques.
From the 36 samples, psychrotrophic bacteria were obtained and classified through conventional culturing techniques, identifying 474 species across 23 genera. At the genus level, the proportions were in the order of Pseudomonas, Acinetobacter, Flavobacterium, Rheinheimera, Psychrobacter, and Enterobacter. The concentration of psychrotrophic bacteria ranged from 1.71 to 3.78 lgCFU/mL, with an average concentration of 2.79 lgCFU/mL. Analysis of the 36 samples using 16SrRNA amplicon sequencing annotated a total of 37 phyla, 90 classes, 214 orders, 358 families, 740 genera and 12 301 species. The top 10 dominant genera in terms of microbial diversity abundance were Acinetobacter, Escherichia, Delftia acidovorans, Aeromonas, Pseudomonas, UCG-005, Streptococcus, Faecalibacterium, Corynebacterium and Flavobacterium.
Substantial variations were observed in the levels of psychrotrophic bacteria, the structure of psychrotrophic bacterial communities and the diversity of psychrotrophic microorganisms in raw milk sourced from various pastures and at various times. This research lays the groundwork for theoretical investigations into the microbial variety of psychrotrophic bacteria found in raw milk in Northern China and supplies data assistance for farms in the area to develop tailored strategies for managing and preventing psychrotrophic bacteria.
PDF
108
15
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
