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Research Progress in Detection Techniques for Mycoplasma bovis
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Beibei JI1, Beibei BAO2, *, Kaifeng CAI3
China Dairy | 2026, (4) : 49 - 56
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China Dairy | 2026, (4): 49-56
DISEASE PREVENTION
Research Progress in Detection Techniques for Mycoplasma bovis
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Beibei JI1, Beibei BAO2, *, Kaifeng CAI3
Affiliations
  • 1.Qingdao Municipal Park Management Service Center, Qingdao Shandong 266000
  • 2.Xinyang Agricultural Comprehensive Administrative Law Enforcement Detachment, Xinyang Henan 464000
  • 3.China Animal Health and Epidemiology Center, Qingdao Shandong 266032
Published: 2026-04-25 doi: 10.12377/1671-4393.26.04.09
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Bovine mastitis is a major disease constraining the healthy development of the dairy industry. Mycoplasma bovis, as one of its key pathogens, is characterized by high contagiosity, strong pathogenicity, and a lack of effective treatments, making its rapid and accurate detection crucial for prevention and control.This paper systematically reviewed the research progress in detection techniques for Mycoplasma bovis, primarily including traditional microbial isolation and culture, serological testing, and DNA-based molecular detection technologies. The advantages and limitations of various methods were analyzed.It is highlighted that molecular detection technologies—especially qPCR—demonstrate significant superiority in sensitivity and specificity, while emerging isothermal amplification technologies such as RPA and LAMP show great potential for field application due to their simplicity, rapidity, and low equipment dependency.This review aimed to provide theoretical support and technical references for the early diagnosis and integrated prevention and control of bovine mastitis.

bovine mastitis  /  mycoplasma bovis  /  microbial isolation and culture  /  serological detection  /  DNA technology
Beibei JI, Beibei BAO, Kaifeng CAI. Research Progress in Detection Techniques for Mycoplasma bovis[J]. China Dairy, 2026 , (4) : 49 -56 . DOI: 10.12377/1671-4393.26.04.09
Year 2026 volume Issue 4
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doi: 10.12377/1671-4393.26.04.09
  • Online Date:2026-08-20
  • Published:2026-04-25
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    1.Qingdao Municipal Park Management Service Center, Qingdao Shandong 266000
    2.Xinyang Agricultural Comprehensive Administrative Law Enforcement Detachment, Xinyang Henan 464000
    3.China Animal Health and Epidemiology Center, Qingdao Shandong 266032
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https://castjournals.cast.org.cn/joweb/zgry/EN/10.12377/1671-4393.26.04.09
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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