[Objective] The detection of folic acid in infant formula is of critical significance for ensuring the healthy growth of infants and young children and for product quality control. Regular participation in detection proficiency testing activities helps enhance the capability level of personnel and provides a guarantee for the improvement of the laboratory's detection ability for folic acid content in infant formula.[Method] This study participated in the proficiency testing project for folic acid detection in infant formula with item number MA-IP-025-042-24-E9326 of the Chinese Academy of Quality and Inspection&Testing. The detection was completed strictly in accordance with the operation instructions provided by the organizer and the detection methods of this laboratory. Based on the specificity and high sensitivity of Lactobacillus rhamnosus to folic acid,in this study,a simple and highly accurate quantitative detection system for folic acid was constructed by using the IfpVitaFast Vitamin B9 Folic Acid test kit in combination with microbiological methods. The folic acid content in the verification sample MA-IP-025-042-24-E9326 was quantitatively detected,and the test results were analyzed and evaluated.[Result] The detected result of folic acid content in sample MA-IP-025-042-24-E9326 was 119.64 μg/100 g,with a Z-value of 0.2,and |Z|≤2. The result was satisfactory.[Conclusion] The test kit method provided accurate detection results,was simple,rapid and efficient to operate,and offers reliable technical support for the analysis of laboratory detection capability of folic acid content in infant formula.

folic acid  /  infant formula  /  microplate reagent kit  /  validation sample