China Dairy
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2025, (9): 108-117
• QUALITY CONTROL •
Exploration of the Current Situation and Future Development Directions of Salmonella Detection Technologies in Dairy Products
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GONG Jinghao, XU Jiawei, LIU Lingfan, Yu Xi, XUE Ruiqi, HOU Xuan, ZHANG Yeni
Affiliations
doi: 10.12377/1671-4393.25.09.16
Outline
The safety of dairy products is crucial to public health.As a common foodborne pathogen in dairy products,the accurate and rapid detection of Salmonella is of vital importance.This review systematically analyzed the current four major testing systems:Although the detection method based on GB 4789.4-2024 National Food Safety Standard - Microbiological testing of food - Salmonella Testing holds the status of a “gold standard”,it has a bottleneck in the testing cycle of 3 to 7 days.Molecular detection methods include conventiona PCR,derived PCR and novel CRISPR/Cas systems. The minimum detection limit can reach 1.00 CFU/mL,but struggle with the problem of interference from live/dead bacteria. Immunoassays include ELISA and lateral flow strips. The minimum detection limit can reach 1.04×102 CFU/mL, but there is matrix interference from various substances in dairy products.Biosensors integrate nanomaterials and aptamers to achieve a minimum detection limit of 5.00 CFU/mL. Its portability makes it suitable for on-site use.Current Salmonella detection technologies have its own advantages and disadvantages. A single technology shows limitations,but multimodal combined technology can overcome these shortcomings. By integrating cutting-edge technologies, it is expected to bring about a revolution in Salmonella detection and enhance public health protection.
Salmonella
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national standard method
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molecular detection method
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immunological detection method
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sensor detection method
宫靖昊, 徐佳薇, 刘灵帆, 于玺, 薛瑞奇, 侯璇, 张业尼.
乳制品中沙门氏菌检测技术现状及未来发展方向探究.
中国乳业,
2025
,
(9)
: 108
-117
.
DOI: 10.12377/1671-4393.25.09.16
GONG Jinghao, XU Jiawei, LIU Lingfan, Yu Xi, XUE Ruiqi, HOU Xuan, ZHANG Yeni.
Exploration of the Current Situation and Future Development Directions of Salmonella Detection Technologies in Dairy Products[J].
China Dairy,
2025
,
(9)
: 108
-117
.
DOI: 10.12377/1671-4393.25.09.16
Year 2025 volume Issue 9
44
8
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Article Info
doi: 10.12377/1671-4393.25.09.16