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To establish an ultra performance liquid chromatography (UPLC) method for determining the concentration of pazopanib in human serum.
Carbamazepine was used as an internal standard (IS), and acetonitrile as a protein precipitant. Separation was performed on an Agilent InfinityLab Poroshell 120 EC-C18 column (2.1 x 50 mm, 1.9 μm) with a mobile phase of 0.1% aqueous formic acid-acetonitrile solution in gradient elution mode. The flow rate was 0.25 mL·min-1, detection wavelength was 268 nm, column temperature was 40 ℃, and injection volume was 25 μL. The specificity of the method, the standard curve and the lower limit of quantification, the accuracy and precision and the stability of the sample were investigated.
Under the established chromatographic conditions, the analyte and internal standard exhibited satisfactory separation without interference from endogenous substances. Pazopanib demonstrated good linearity within the concentration range of 0.5-100 μg·mL-1. The standard curve was y=63.37×10-2x-0.49×10-2 (r2=0.999 9) and the lower limit of quantification can reach 0.25 μg·mL-1. The relative standard deviations (RSD) for both intra-day. and inter-day precision were less than 12.02%, with extraction recovery rates exceeding 86.44%. Stability was good, with RSD below than 8.80%.
The established UPLC method is simple, selective, sensitive, accurate, and reliable for determining pazopanib in human serum.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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