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Rapid detection of cotinine in urines by colloidal gold immunochromatography
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Jun-min DONG1, 4, Long LIU2, 4, Xiao-hua HAO1, 4, Man ZHANG3, 4, 5
Chinese Journal of Clinical Pharmacology | 2025, 41(24) : 3524 - 3529
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Chinese Journal of Clinical Pharmacology | 2025, 41(24): 3524-3529
• Research Method •
Rapid detection of cotinine in urines by colloidal gold immunochromatography
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Jun-min DONG1, 4, Long LIU2, 4, Xiao-hua HAO1, 4, Man ZHANG3, 4, 5
Affiliations
  • 1.Phase Ⅰ Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 2.Medical Examination Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 3.Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 4.Peking University Ninth School of Clinical Medicine, Beijing 100038, China
  • 5.Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing 100038, China
Published: 2025-12-28 doi: 10.13699/j.cnki.1001-6821.2025.24.012
Outline
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Objective

To establish a colloidal gold immunochromatographic method for the rapid detection of cotinine in urines.

Methods

To prepare the colloidal gold-labeled antibody probe, optimization of the pH value and the amount of antibody labeling (COT-Mab) was carried out. Following this, the coating concentrations of the cotinine antibody (COT-BSA) for the test (T) line and the sheep anti-mouse immunoglobulin G (IgG) for the control (C) line were also optimized. Subsequently, a colloidal gold immunochromatographic strip was assembled. Finally, the sensitivity of the strip was evaluated.

Results

The optimal conditions established for the colloidal gold immunochromatographic strip were as followings, pH value of 6.5, COT-Mab concentration of 10.00 μg·mL-1, COT-BSA concentration of 0.10 mg·mL-1, and sheep anti-mouse IgG concentration of 1.00 mg·mL-1. The visual detection limit and the cut-off value were 0.01 and 0.20 μg·mL-1, respectively. Within 15 minutes, this method enabled qualitative analysis of cotinine in urines.

Conclusion

The method has good specificity and accuracy, as well as convenient operation and excellent reproducibility, which can be used for on-site detection and rapid initial screening of smokers and non-smokers during drug clinical trials.

cotinine  /  colloidal gold immunochromatographic assay  /  urine  /  qualitative detection
Jun-min DONG, Long LIU, Xiao-hua HAO, Man ZHANG. Rapid detection of cotinine in urines by colloidal gold immunochromatography[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (24) : 3524 -3529 . DOI: 10.13699/j.cnki.1001-6821.2025.24.012
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Year 2025 volume 41 Issue 24
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Article Info
doi: 10.13699/j.cnki.1001-6821.2025.24.012
  • Receive Date：2025-11-01
  • Online Date：2026-08-05
  • Published：2025-12-28
Article Data
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History
  • Received：2025-11-01
Funding
Affiliations
    1.Phase Ⅰ Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
    2.Medical Examination Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
    3.Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
    4.Peking University Ninth School of Clinical Medicine, Beijing 100038, China
    5.Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing 100038, China
References
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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