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To explore the main active components, potential targets and mechanisms of Yinpu Jiedu tablets in the treatment of diabetic renal inflammation by using network pharmacological methods and molecular docking techniques.
The active ingredients of Yinpu jiede tables and therapeutic targets for diabetic kidney inflammation were retrieved from databases including TCMSP, PubChem, SwissTargetPrediction and GeneCards. A protein-protein interaction (PPI) network was constructed using Venny, STRING, Metascape, Bioinformatics and Cytoscape, followed by gene enrichment analysis. Molecular docking was performed using AutoDock, and the results were visualized with PyMOL. Furthermore, a lipopolysaccharide (LPS)-induced cellular model of diabetic kidney inflammation was established. The experiment included control group, model group (4 μmol·L-1 LPS) and experimantol group (30 μg·mL-1 Yinpu jiede tables). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to measure the messenger ribonucleic acid (mRNA) relative expression levels of AKT1 serine/threonine kinase 1(Akt1), tumor protein p53(Tp53), tumor necrosis factor-α (Tnf-α) and interleukin-6 (IL-6) to validate the network pharmacology predictions.
A total of 30 active ingredients and 435 potential targets of Yinpu jiede tables were identified. The core active ingredients included quercetin, luteolin, acacetin, morin and pelargonin. Intersection analysis between disease-related targets and drug-targets yielded 71 key genes, from which 4 core targets, inducling AKT1, TNF-α, IL-6 and TP53. Molecular docking results indicated strong binding affinity between the core active ingredients and the core targets. gene ontolog (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses suggested that Yinpu jiede tables exerts its therapeutic effects on diabetic kidney inflammation primarily through pathways such as pathways in cancer, the JAK-STAT signaling pathway, the AMPK signaling pathway and the Wnt signaling pathway. Cellular experiments confirmed that LPS induction significantly upregulated the mRNA expression of Akt1, Tp53, TNF-α, and IL-6 in podocytes, while treatment with Yinpu jiede tables effectively reversed this trend, restoring their expression to near-normal levels, thereby validating its regulatory role on the predicted targets and inflammatory pathways.
Yinpu jiede tables may act on signal pathways such as JAK-STAT, AMPK, and Wnt through core targets such as AKT1, TNF, IL-6, and TP53, and exert therapeutic effects on diabetic kidney inflammation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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