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To establish a high performance liquid chromatography method for the determination of arecoline in rat plasma, to study the pharmacokinetic behavior of arecoline in rats, and to provide a scientific basis for the pharmacodynamic substance basis of arecoline.
SD rats were given low, medium and high (10, 20 and 30 mg·kg-1) aqueous solutions of arecine by gavage, and blood was collected from the fundus venous plexus at different time points, and the supernatant was extracted by ethyl acetate and then injected for analysis. Puerarin was used as the internal standard to detect the content of arecine in plasma by HPLC, the mobile phase was acetonitrile-methanol-0.1% triethylamine aqueous solution (6∶16∶78, pH=4.5), isocratic elution, flow rate 1.0 mL·min-1, detection wavelength 215 nm, column temperature 30 ℃, and the pharmacokinetic parameters were calculated by DAS2.0 software.
The linear relationship between arecaine in 0.5-32.0 μg·mL-1 was good, the standard curve was y=0.0512x-0.0326(r=0.9960), the lower limit of quantification was 0.5 μg·mL-1, and the precision, repeatability, stability and recovery test results were good. The main pharmacokinetic parameters of arecaine in the low-, medium-, and high-dose groups were (1.36±0.21), (2.15±0.72) and (2.75±0.69) mg·L-1, and the area under the curve (AUC0-t) was (3.04±0.24), (3.87±1.02) and (4.49±0.76) mg·L-1·h, and the elimination half-lives (t1/2) were (3.67±1.73), (2.88±1.66) and (3.12±1.86) h.
The method is simple and rapid, suitable for the pharmacokinetic study of arecoline in rat plasma.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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