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To explore the effects of ropivacaine on the cyclic guanosine monophosphate synthase interferon gene stimulating factor (cGAS/STING) pathway in glial cells of septic encephalopathy (SAE) model mice, and its related mechanisms of action.
Mice were randomly divided into blank group, model group, experimental group and control group, with 15 mice in each group. Except for the blank group and control group, the other two groups were used to establish sepsis mouse models by intraperitoneal injection of lipopolysaccharide. The experimental group and control group were intraperitoneally injected with 0.3 mg·kg-1·d-1 ropivacaine, while the blank group and model group were intraperitoneally injected with an equal volume of 0.9% NaCl. The four groups of mice were administered once a day for 7 consecutive days. The Morris water maze was used to detect the cognitive function of the mouse brain, real-time fluorescent quantitative polymerase chain reaction was used to detect the mRNA levels of brain inflammatory factors, Western blot was used to detect the expression levels of brain cGAS and STING proteins, and immunofluorescence staining was used to detect the expression of apoptosis-related proteins.
The exploration time proportions of the blank group, model group, experimental group and control group were (0.48±0.10)%, (0.23±0.05)%, (0.37±0.04)% and (0.46±0.07)%, respectively; the platform crossing times were (4.67±0.48), (1.03±0.11), (2.50±0.65) and (4.99±0.52) times, respectively; the escape latency time was (11.92±0.98), (12.49±1.22), (11.17±1.43) and (12.21±1.52) min, respectively; the relative expression levels of interleukin-1β (IL-1β) mRNA were 1.00±0.10, 3.80±0.28, 1.87±0.13 and 1.06±0.29, respectively; the relative expression levels of IL-18 mRNA were 1.00±0.12, 1.61±0.27, 1.06±0.08 and 0.97±0.05, respectively; the relative expression levels of tumor necrosis factor (TNF)-α mRNA were 1.00±0.13, 2.02±0.18, 1.05±0.09 and 1.13±0.11, respectively; the relative expression levels of IL-6 mRNA were 1.00±0.08, 1.56±0.11, 1.05±0.09 and 0.93±0.06, respectively; the relative expression levels of cGAS protein were 1.00±0.08, 1.92±0.19, 1.42±0.11 and 1.03±0.10, respectively; the relative expression levels of STING protein were 1.00±0.12, 2.01±0.22, 1.18±0.09 and 1.08±0.16, respectively; the relative fluorescence intensities of cGAS protein were 1.00±0.18, 1.88±0.15, 1.57±0.09 and 1.03±0.11, respectively; the relative fluorescence intensities of STING were 1.00±0.16, 1.79±0.26, 1.14±0.06 and 1.20±0.29, respectively; the relative fluorescence intensities of caspase-3 protein were 1.00±0.12, 1.51±0.08, 1.23±0.10, and 1.11±0.15, respectively. The above indexes of the experimental group and the control group were statistically different from the model group (all P<0.05).
Ropivacaine can alleviate apoptosis of glial cells in the hippocampus of SAE mice by inhibiting the cGAS/STING pathway, and has a protective effect on the brain of SAE mice.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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