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To investigate the effect of Jinfu’an decoction on the apoptosis of non-small cell lung cancer cells (A549) by regulating phosphatidylinositol 3-kinase/ protein kinase B/ mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway mediated autophagy.
A549 cells were randomly divided into blank group, control group, combined group and experimental -L, -M, -H groups. The blank group was treated with 10% blank serum. The control group was treated with 20 μmol·L-1 PI3K specific activator (740Y-P). The experimental-L, -M, -H groups were treated with 5%, 10% and 20% Jinfu ’an decoction containing serum, respectively. The combined group was treated with 20% Jinfu ’an decoction serum +20 μmol·L-1 740Y-P. Flow cytometry was used to detect the apoptosis rate. Western blot was used to detect the expression levels of microtubule-associated protein 1A/1B -light chain 3 (LC3), sequestosome-1 (p62), Akt and mTOR.
The apoptosis rates of experimental -H group, combined group, control group and blank group were (31.48±1.35)%, (14.91±1.26)%, (3.30±0.45)% and (7.73±0.50)%, respectively; the relative expression levels of LC3 Ⅱ/Ⅰ protein were 1.13±0.10, 0.77±0.06, 0.31±0.04 and 0.50±0.04, respectively; the relative expression levels of p62 protein were 0.32±0.03, 0.56±0.05, 1.07±0.06 and 0.83±0.06, respectively; the relative expression levels of p-Akt/Akt protein were 0.33±0.04, 0.80±0.07, 0.98±0.09 and 0.52±0.03, respectively; the relative expression levels of p-mTOR/mTOR protein were 0.53±0.04, 0.84±0.06, 1.14±0.08 and 1.01±0.06, respectively. The above indexes of the experimental -H group were statistically different from those of the combination group and the blank group (all P<0.05).
Jinfu’an decoction can promote the apoptosis of A549 cells, and its mechanism may be related to the inhibition of PI3K/Akt/mTOR signaling pathway and the activation of cell autophagy.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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