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To explore the effects of Wu Wei Han bamboo slips “Yufang” on kidney injury in spontaneously hypertensive rats and its mechanism of action.
To analyze the differential genes between normal kidney tissues and hypertensive kidney tissues in GSE37460, a hypertensive kidney disease dataset in GEO database, and to explore the mechanism of hypertensive kidney injury; Spontaneously hypertensive rats (SHR) were randomly divided into model group (equal volume of distilled water), control group (7.59 mg·kg-1 valsartan) and experimental-L, -M, -H groups (7.15, 14.29, 28.58 g·kg-1 “Yufang” granules), with 10 rats in each group; additionally, Wistar-Kyoto (WKY) rats were taken as a normal group (equal volume of distilled water). Six groups were gavaged once a day for 8 consecutive weeks. The levels of urinary microalbumin (mAlb), N-acetyl-β-D-glucosaminidase (NAG) and serum thromboxane (TXB2), 6-keto-prostaglandin F1 α (6-keto PGF1α) were detected by enzyme linked immunosorbent assay. Western blot was used to detect the expression levels of hypoxia inducible factor-1 α (HIF-1α), vascular endothelial growth factor (VEGF) and transmembrane receptor protein 1 (Notch1) in rat kidney.
The data set GSE37460 contained 155 differential genes, 94 of which were up-regulated and 61 were down regulated and pathway enrichment involves pathways such as HIF-1α/VEGF/Notch1. The urinary mAlb levels of normal, model, control and experimental -H groups were (60.83±3.81), (159.60±20.65), (70.45±3.97) and (79.72±9.01) μg·L-1, respectively; the urinary NAG levels were (34.37±6.92), (91.30±9.05), (44.65±10.09) and (54.79±2.87) ng·L-1, respectively; the contents of TXB2 in serum were (78.58±3.78), (107.80±5.16), (80.92±4.02) and (85.65±2.17) pg·mL-1, respectively; the serum 6-keto PGF1α levels were (121.50±3.45), (85.05±2.92), (108.30±2.96), (101.90±3.08) pg·mL-1, respectively; the relative expression levels of HIF-1 α protein in rat kidney were 0.56±0.03, 1.25±0.02, 0.77±0.01 and 0.83±0.02, respectively; the relative expression levels of VEGF protein were 0.24±0.02, 1.24±0.03, 0.81±0.04 and 0.95±0.02, respectively; the relative expression levels of Notch1 protein were 0.33±0.03, 1.02±0.01, 0.50±0.04 and 0.68±0.01, respectively; there were significant differences in the above indexes between the control and experimental -H groups and the model group (all P<0.05).
The Wuwei Han bamboo slips “Yufang” can improve hypertension-related kidney damage and reduce kidney fibrosis, and its mechanism may be related to the inhibition of the HIF-1α/VEGF/Notch1 signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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