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Research on GLPS alleviated H2O2-induced ferroptosis in mouse hippocampal neurons by regulating Nrf2/HO-1 signaling pathway
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Yan-qing LI1, Zhi-chao YANG1, Li-juan SONG1, Qing WANG1, Han-bin WANG2, Li-zhi YANG2, Bao-guo XIAO3, Cun-gen MA1
Chinese Journal of Clinical Pharmacology | 2025, 41(6) : 805 - 810
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Chinese Journal of Clinical Pharmacology | 2025, 41(6): 805-810
• Clinical and Basic Bridging Research •
Research on GLPS alleviated H2O2-induced ferroptosis in mouse hippocampal neurons by regulating Nrf2/HO-1 signaling pathway
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Yan-qing LI1, Zhi-chao YANG1, Li-juan SONG1, Qing WANG1, Han-bin WANG2, Li-zhi YANG2, Bao-guo XIAO3, Cun-gen MA1
Affiliations
  • 1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China
  • 2.Shanxi Guorun Pharmaceutical Co., Ltd, Datong 038100, Shanxi Province, China
  • 3.State Key Laboratory of Neurobiology, Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China
Published: 2025-03-28 doi: 10.13699/j.cnki.1001-6821.2025.06.011
Outline
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Objective

To investigate the effect of ganoderma lucidum polysaccharides (GLPS) on ferroptosis of hippocampal neuronal (HT22) cell lines induced by hydrogen peroxide (H2O2) and its mechanism.

Methods

The H2O2-induced HT22 cell injury model was first established in vitro. HT22 cells were divided into normal group (conventional culture), H2O2 group (200 μmol·L-1 H2O2), GLPS group (200 μmol·L-1 H2O2+100 μg·mL-1 GLPS), H2O2+Fin56 group (200 μmol·L-1 H2O2+5 μmol·L-1 Fin56), H2O2+Fin56+GLPS group (200 μmol·L-1 H2O2+5 μmol·L-1 Fin56+100 μg·mL-1 GLPS). The oxidative stress markers were detected by enzyme-linked immunosorbent assay (ELISA); the protein expression levels of iron death markers, nuclear factor erythroid2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot.

Results

The contents of malondialdehyde (MDA) in normal group, H2O2 group, GLPS group, H2O2+Fin56 group and H2O2+Fin56+GLPS group were (5.56±0.61), (15.53±0.83), (6.51±0.29), (20.92±1.03) and (13.65±0.70) nmol·mL-1; the contents of superoxide dismutase (SOD) were (37.93±1.37), (22.48±0.59), (37.81±2.56), (16.10±2.28) and (22.21±2.99) μg·mL-1; Ferritin protein expression levels were 1.52±0.03, 1.22±0.07, 1.42±0.06, 0.64±0.02 and 0.93±0.13; GPX4 protein expression levels were 3.29±0.34, 1.91±0.18, 2.99±0.22, 1.13±0.07 and 2.45±0.05; Nrf2 protein expression levels were 0.90±0.01, 2.24±0.02, 2.97±0.07, 2.45±0.07 and 2.76±0.11; HO-1 protein expression levels were 1.58±0.02, 1.76±0.04, 2.29±0.06, 1.67±0.02 and 2.03±0.06, respectively. H2O2 group was compared with normal group and H2O2+Fin56 group, GLPS group was compared with H2O2 group, H2O2+Fin56+GLPS group was compared with H2O2+Fin56 group and GLPS group, the differences were statistically significant (P<0.05, P<0.01).

Conclusion

GLPS can improve lipid peroxide accumulation and iron metabolism disorder induced by H2O2 in HT22 cells by activating Nrf2/HO-1 pathway, thereby inhibiting the occurrence of ferroptosis.

ganoderma lucidum polysaccharides  /  hippocampal neuronal cell line  /  hydrogen peroxide  /  ferroptosis  /  lipid peroxidation  /  nuclear factor erythroid2-related factor 2/heme oxygenase-1 pathway
Yan-qing LI, Zhi-chao YANG, Li-juan SONG, Qing WANG, Han-bin WANG, Li-zhi YANG, Bao-guo XIAO, Cun-gen MA. Research on GLPS alleviated H2O2-induced ferroptosis in mouse hippocampal neurons by regulating Nrf2/HO-1 signaling pathway[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (6) : 805 -810 . DOI: 10.13699/j.cnki.1001-6821.2025.06.011
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Year 2025 volume 41 Issue 6
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Article Info
doi: 10.13699/j.cnki.1001-6821.2025.06.011
  • Receive Date：2024-10-10
  • Online Date：2026-08-04
  • Published：2025-03-28
Article Data
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History
  • Received：2024-10-10
Funding
Affiliations
    1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine/Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, Shanxi Province, China
    2.Shanxi Guorun Pharmaceutical Co., Ltd, Datong 038100, Shanxi Province, China
    3.State Key Laboratory of Neurobiology, Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China
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表12种不同金属材料的力学参数
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Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
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Genus		 种数
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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