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To explore the effect and mechanism of piperlongumine on hepatic steatosis induced by sleep deprivation.
Mice were randomly divided into control group (normal feeding), model group (sleep deprivation treatment), low-, middle- and high dose groups (2.5, 5.0 and 10.0 mg·kg-1 piperlongumine was given on the basis of sleep deprivation), and the mice were given continuous gavage for 14 days. Liver index was detected after 14 days of continuous gavage. Western blot assay was used to detect protein expression in hepatic tissue, lipid levels in hepatic tissue were detected by kit, and reactive oxygen species (ROS) levels in hepatic tissue were detected by dihydroethidium (DHE).
The liver index of control group, model group, and high-dose group were (2.99±0.40)%, (4.32±0.17)% and (3.30±0.37)%, respectively; triglyceride (TG) levels were (0.16±0.011), (0.29±0.02) and (0.17±0.02) mmol·mgprot-1, respectively; fatty acid synthase (FAS) protein levels were 0.31±0.03, 0.69±0.08 and 0.35±0.03, respectively; hypoxia-inducing factor-1 α (HIF-1α) protein levels were 0.38±0.04, 0.94±0.08 and 0.44±0.04, respectively; the protein levels of adaptor p66Shc (p66Shc) were 0.21±0.03, 0.82±0.07 and 0.37±0.04, respectively; ROS levels were 1.00±0.09, 4.72±0.44 and 1.30±0.07, respectively. The above indexes in the model group were significantly different from those in the control group, and the above indexes in the high-dose groups were significantly different from those in the model group (all P<0.05).
Piperlongumine can improve liver steatosis induced by sleep deprivation in mice, which may be related to the regulation of HIF-1α/p66Shc signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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