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To investigate the protective effect of Danzhi Jiangtang capsule on kidney of db/db mice with diabetic kidney disease (DKD) and its effects on the autophagy-lysosomal pathway.
The db/db mice were randomly divided into model group (0.2 mL·d-1 0.9% NaCl), positive control group (45 mg·kg-1·d-1 irbesartan) and experimental-H, -M, -L groups (1 800, 900 and 450 mg·kg-1·d-1 Danzhi Jiangtang capsule), and another db/m mice were taken as blank group (0.2 mL·d-1 0.9% NaCl), which were continuously infused for 8 weeks. The expression levels of sequestosome1 (P62), microtubule-associated protein light chain 3 (LC3), transcription factor EB (TFEB), lysosomal associated membrane protein 1 (LAMP-1) and cathepsin D (CTSD) were detected in renal tissues by Western blot.
The relative expression levels of P62 protein in blank group, model group, positive control group and experimental-H,-M,-L groups were 1.00±0.04, 7.66±0.27, 3.52±0.11, 2.21±0.09, 2.86±0.10 and 4.05±0.16; the LC3Ⅱ/Ⅰ levels were 1.00±0.02, 0.17±0.01, 0.71±0.08, 0.86±0.05, 0.80±0.04 and 0.73±0.06; the TFEB protein levels were 1.00±0.01, 0.17±0.01, 0.30±0.01, 0.63±0.01, 0.45±0.01 and 0.42±0.01; the LAMP-1 protein levels were 1.00±0.01, 0.28±0.02, 0.42±0.01, 0.77±0.00, 0.55±0.03 and 0.40±0.02; the CTSD protein levels were 1.00±0.04, 0.27±0.03, 0.47±0.04, 0.73±0.02, 0.63±0.01 and 0.52±0.02. The differences between the above indexes in the experimental-H, -M, -L groups and the model group were statistically significant (all P<0.01).
Danzhi Jiangtang capsule can improved renal damage in db/db mice, which may be related to the repair of autophagy-lysosome pathway, accelerate clearance of abnormal proteins and degradation of organelles.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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