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Probing whether salivary acid-binding immunoglobulin-like agglutinin-15 (Siglec-15) promotes hepatocellular carcinoma proliferation and migration by regulating the signal transducer and activator of transcription 1/3 (STAT1/STAT3) pathway.
SMMC-7721 and BEL-7402 cells were divided into empty vector groups: S-NC and B-NC groups (NC siRNA was transfected into SMMC-7721 and BEL-7402 cell, respectively), Siglec-15 knockdown expression group: S-si-Siglec-15-3 and B-si-Siglec-15-3 groups (Siglec-15-3 siRNA was transfected into SMMC-7721 and BEL-7402 cell, respectively). The proliferation ability of the cells were detected by methyl thiazolyl tetrazolium (MTT); the longitudinal and lateral migration ability of the cells were detected by Transwell and scratch assay; the expression of STAT3, phosphorylation of STAT3 (p-STAT3), STAT1, phosphorylation of STAT1 (p-STAT1), Siglec-15 was detected by Western blot.
Cell viability values at 24 h in the S-NC group, S-si-Siglec-15-3 group, B-NC group and B-si-Siglec-15-3 group were 0.93±0.01, 0.87±0.03, 0.96±0.03 and 0.83±0.02, respectively; the vertical migration rates of cells were 100.00%, 36.40%, 100.00% and 52.20%, respectively; the healing rate of cell scratches at 24 h were (0.76±0.02)%, (0.53±0.02)%, (0.44±0.02)% and (0.36±0.03)%, respectively; STAT3 protein expression levels were 1.11±0.05, 0.66±0.05, 1.07±0.14 and 0.49±0.04, respectively; p-STAT3 protein expression levels were 1.07±0.04, 0.16±0.06, 0.78±0.05 and 0.32±0.03, respectively; STAT1 protein expression levels were 1.32±0.04, 0.71±0.04, 0.91±0.03 and 0.61±0.04, respectively; p-STAT1 protein expression levels were 0.99±0.04, 0.29±0.05, 0.67±0.04 and 0.20±0.06, respectively; Siglec-15 protein expression levels were 1.51±0.04, 0.93±0.05, 0.73±0.02 and 0.12±0.01, respectively. The differences between the S-si-Siglec-15-3 group and the S-NC group, between the B-si-Siglec-15-3 group and the B-NC group were statistically significant in all comparisons (P<0.05, P<0.01, P<0.001).
Siglec-15 promotes the proliferation and migration ability of hepatocellular carcinoma cells through the STAT1/STAT3 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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