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To investigate the effects of baicalein on apoptosis and immune escape of laryngeal carcinoma cells and its mechanism.
Human laryngeal carcinoma TU686 cells were randomly divided into control group(routine culture), baicalein group (50 μmol·L-1 baicalein), Vector group (transfected with Vector+50 μmol·L-1 baicalein) and CD47 group [transfected with differentiation cluster 47(CD47) +50 μmol·L-1 baicalein]. Cell proliferation and apoptosis were detected by 5-acetylidene-2′-deoxyuridine (Edu) method and Annexin V-FITC/PI double staining method. The expression of proliferative and apoptotic proteins were detected by Western blot. Human peripheral lymphocytes were co-cultured with cells in each group to detect the killing rate of TU686 cells. The phagocytosis rate of TU686 cells was observed.
The Edu positive cell rates in control group, baicalein group, Vector group and CD47 group were (36.24±2.74)%, (13.33±1.93)%, (14.22±1.48)% and (27.80±2.74) %, respectively; the apoptosis rates were (3.94±0.40)%, (28.40±1.76)%, (26.60±1.41)% and (12.33±0.84)%, respectively; the relative expressions of CD47 protein were 1.00±0.11, 0.42±0.07, 0.44±0.06 and 1.21±0.12, respectively; the relative expression levels of CyclinD1 protein were 0.86±0.08, 0.37±0.05, 0.36±0.04 and 0.74±0.08, respectively; the relative expression levels of cleaved cysteine aspartate proteinase-7(Cl-Caspase-7) protein were 0.35±0.04, 0.99±0.12, 1.00±0.11 and 0.50±0.06, respectively; the killing rates of human peripheral lymphocytes to TU686 cells (1∶1 ratio) were (10.61±1.19)%, (32.02±2.27)%, (31.86±2.06)% and (14.39±1.07)%, respectively; the phagocytosis rates were (6.35±0.71)%, (11.99±1.64)%, (12.03±0.76)% and (7.06±0.57)%, respectively. The above indicators, baicalein group compared with the control group, Vector group compared with CD47 group, the differences were statistically significant (P<0.05, P<0.01, P<0.001).
Baicalein may induce the apoptosis of laryngeal carcinoma cells and inhibit the immune escape of laryngeal carcinoma cells by inhibiting the expression of CD47, thus slowing down the progression of laryngeal carcinoma.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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