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To observe the effect of Tetrahydropalmatine on the epidermal growth factor receptor / phosphatidylinositol 3-kinase/protein kinase B (EGFR/PI3K/AKT) signaling pathway in endometriosis dysmenorrhoea SD rats.
The endometriosis dysmenorrhoea model was replicated by auto-transplantation in 60 female SD rats of SPF grade. After successful modelling, the rats were randomly divided into model group, control group and experimental -L, -M, -H groups according to body mass, with 12 rats in each group; and another 12 normal rats were taken as the blank group. The blank and model groups were given 10 mL·kg-1 0.9% NaCl by gavage, and the control group was given 0.25 mg·kg-1 progesterone suspension by gavage. The experimental-L, experimental-M, experimental-H groups were given 10, 20 and 40 mg·kg-1 Tetrahydropalmatine suspension by gavage, respectively. Six groups of rats were administered once daily for 4 weeks. After the last administration, 2 U of oxytocin was given to induce contractions, and the rats were observed for torsional responses. The serum levels of tumor necrosis factor -α (TNF-α), epidermal growth factor (EGF), and EGFR were measured by enzyme-linked immunosorbent assay in each group of rats, and the expression levels of EGFR, PI3K and AKT proteins in ectopic endometrial tissues were detected by Western blotting.
The number of twists in the experimental -M, -H groups and control group, model group, blank group were 9.25±1.42, 8.33±1.88, 11.17±2.41, 28.67±2.15 and 0; the inhibition rates were 67.74%, 70.95%, 61.05%, 0 and 0; serum TNF-α levels were (281.96±13.06), (278.75±10.39), (282.12±14.47), (303.59±9.09) and (274.26±15.33) ng·L-1; serum EGF levels were (313.82±13.23), (308.26±10.90), (311.39±19.52), (336.04±17.60) and (301.67±27.17) pg·mL-1; serum EGFR levels were (315.71±7.56), (311.43±11.04), (313.75±7.26), (333.56±14.22) and (307.86±17.94) ng·L-1; the relative expression levels of EGFR protein were 1.60±0.39, 1.26±0.30, 1.40±0.40, 2.08±0.41 and 1.00±0.22; the relative expression levels of PI3K protein were 1.60±0.42, 1.21±0.18, 1.27±0.24, 2.01±0.41 and 1.00±0.19; the relative expression levels of AKT protein were 1.27±0.18, 1.11±0.10, 1.27±0.16, 1.64±0.28 and 1.00±0.07, respectively. Statistically significant differences were found between the above indicators in the experimental -M, -H groups compared to the model group (P<0.01, P<0.05).
Tetrahydropalmatine may play a therapeutic role in endometriosis dysmenorrhoea by interfering with the EGFR/PI3K/AKT signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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