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To establish a ultra-high performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) method for determining the concentration of sotagliflozin in rat plasma and apply it to pharmacokinetic studies in rats.
Electrospray negative ion multi-reaction ion detection was used. Chromatographic column: EXT-C18 (2.1 mm×100.0 mm, 2.7 μm); column temperature: 45 ℃; mobile phase: 5 mmol·L-1 ammonium acetate aqueous solution-acetonitrile; flow rate: 0.35 mL·min-1; ion pairs: sotagliflozin m/z 483.3→315.1, dapagliflozin m/z 467.4→329.2; injection volume: 6 μL, plasma samples were processed using methyl tert-butyl ether liquid-liquid extraction. Six male SD rats were administered a single oral dose of sogliflozin at 40 mg·kg-1, and detected the concentration of sogliflozin in plasma. Pharmacokinetic parameters were calculated using Drug And Statistics (DAS) 2.1.1.
Sotagliflozin showed good linearity within the range of 5-2 000 ng·mL-1, with intra-day and inter-day precision both less than 15%. The recovery rate, matrix effect, and stability were all within the specified range. Pharmacokinetic parameters: Cmax was (3 716.67±568.28) ng·mL-1, tmax was (1.00±0.32) h, t1/2 was (2.28±0.45) h, AUC0-t was (1.70×104±2 075.87) ng·mL-1·h.
This study established a method for determining the concentration of sotagliflozin in rat plasma, which is characterized by high sensitivity, rapid detection, and good repeatability. It is suitable for the determination of sotagliflozin concentration in plasma and pharmacokinetic studies.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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