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To study the effects of Hedysarum polysaccharides polysaccharide (HPS) on the farnesoid X receptor (FXR)-fibroblast growth factor-19(FGF19) signaling pathway of diabetes rats.
Twelve Wistar male rats were randomly selected as the normal group, and the other rats were fed with a single intraperitoneal injection of streptozotocin (50 mg·kg-1 STZ) and a high sugar and high-fat diet to replicate the diabetes rat model. Model rats were randomly divided into model group, positive control group (given 400 mg·kg-1·d-1 suspension of Bifidobacterium quadruplex live bacterial tablets by gavage), experimental-H, -M, -L groups (given 200, 100, and 50 mg·kg-1·d-1 doses of HPS suspension by gavage); normal group, and model group were given equal volume of purified water by gavage once a day for 8 consecutive weeks. Glucose (Glu) was detected by a blood glucose meter; and serum total glyceride (TG) and total cholesterol (TC) were detected by enzyme-linked immunosorbent assay reagent kit; the expressions of FXR、fibroblast growth factor receptors 4 (FGFR4) relative mRNA expression level and protein were detected by real-time fluorescence quantitative polymerase chain reaction method and Western blot.
The Glu concentrations in the normal group, model group, positive control group, and experimental-H groups were (7.66±0.61), (29.25±1.64), (23.31±3.02) and (19.31±5.13) mmol·L-1, respectively; the TG content were (957.00±113.73), (1 345.00±246.44), (958.00±96.53) and (964.00±130.22) μmol·L-1, respectively; the TC content were (161.65±4.53), (302.19±5.35), (236.09±5.14) and (165.58±2.58) μmol·L-1, respectively; the expression of FXR relative mRNA expression level were 1.00±0.06, 0.48±0.02, 0.67±0.04 and 0.92±0.04, respectively; the expression of FGFR4 relative mRNA expression level were 1.00±0.04, 0.17±0.01, 0.48±0.04 and 0.41±0.03; respectively. The above indexes of the model group were compared with the control group, and the above indexes of the control group and the experimental-H group were compared with the model group, and the differences were statistically significant (all P<0.01).
HPS improves blood sugar, lowers blood lipids, and protects liver and intestinal tissues, possibly by regulating the FXR-FGF19 signaling pathway in intestinal tissue, and regulating bile acid synthesis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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