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To investigate the effect and mechanism of methyl oxofulnonone A (META) on high glucose (HG)-induced H9c2 cell injury.
H9c2 cells were divided into control group (normal culture), model group (55 mmol·L-1 glucose) and experimental -L, -M, -H groups (55 mmol·L-1 glucose+12.5, 25.0, 50.0 μmol·L-1 META). Cell viability was detected by cell counting kit-8; intracellular reactive oxygen species (ROS) level was detected by DCFH-DA fluorescent probe; intracellular adenosine triphosphate (ATP) content was detected by luciferase; and autophagy-related protein expression was detected by Western blotting.
The optical density values of 72-hour cells in the control group, model group and experimental -M, -H groups were 0.91±0.03, 0.61±0.01, 0.69±0.02 and 0.72±0.03; the ROS levels were (40.75±1.53)%, (43.73±1.30)%, (30.87±1.27)% and (28.28±1.43)%; the ATP contents were (8.16±0.71), (4.03±0.29), (5.29±0.31) and (5.83±0.31) nmol·mg-1; the relative expression levels of autophagy-related gene 5 protein were 1.05±0.06, 1.46±0.09, 0.98±0.11 and 0.89±0.09; the relative expression levels of ubiquitin-binding protein were 1.05±0.10, 0.55±0.13, 0.89±0.04 and 0.98±0.04; the ratios of microtubule-associated protein 1 light chain 3 Ⅱ/Ⅰ protein were 1.09±0.09, 1.82±0.05, 1.67±0.29 and 1.09±0.15, respectively. Among the above indicators, there were statistically significant differences between the model group and the control and experimental -M, -H groups (P<0.05, P<0.01).
META significantly ameliorates H9c2 cardiomyocyte damage caused by high glucose, ameliorates oxidative stress, protects mitochondrial respiration and inhibits autophagy.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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