PDF
Full
Outline
To investigate the inhibition of NG108-15 cells proliferation by Xanthohumol (XN) and its effects on reactive oxygen species (ROS), extracellular signal-regulated kinase (ERK) pathway and cell cycle.
NG108-15 cells were divided into control group (conventional culture), experimental-L group, experimental-M group, experimental-H group (20, 40, and 60 μmol·L-1 XN), NAC combined with XN experimental group [10 μmol·L-1 N-acetylcysteine (NAC)+40 μmol·L-1 XN] and ERK inhabitor combined with XN experimental group (20 μmol·L-1 ERK inhibitor PD98059+40 μmol·L-1 XN). Cell proliferation capacity was detected by cell counting kit 8 (CCK-8) and trypan blue staining assay; ROS levels were detected by ROS kit; cell cycle and apoptosis levels were detected by flow cytometry; protein relative expression levels of ERK, p-ERK and Caspase 3 were detected by Western blot.
The cell proliferation activities of the control group and the experimental-L group, experimental-M group and experimental-H group were (99.89±1.52)%, (87.57±6.23)%, (51.96±3.08)% and (37.11±1.57)%, respectively; the cell death rates were (4.65±1.57)%, (15.54±1.94)%, (41.06±2.77)% and (65.22±5.81)%, respectively; the average fluorescence intensities of ROS in the control group, the experimental-M group and the NAC combined with XN experimental group were 176.51±25.17, 716.48±95.15 and 543.80±48.91. The relative expression levels of p-ERK protein in the control group, the experimental-M group, and the ERK inhibitor combined with XN experimental group were 0.54±0.04, 1.13±0.18 and 0.31±0.33; the proportions of G2 phase cells were (15.56±1.43)%, (20.31±2.17)% and (16.14±0.81)%, respectively. Compared with the control group, the above indicators in the experimental-L, -M, -H groups showed statistically significant differences (all P<0.001). Compared with the experimental-M group, the control group and the NAC combined with XN experimental group showed statistically significant differences (P<0.05, P<0.01, P<0.001). Compared with the experimental-M group, the ERK inhibitor combined with XN experimental group showed statistically significant differences (P<0.05, P<0.01, P<0.001).
Xanthohumol demonstrates potent inhibition of NG108-15 cells proliferation, with its molecular mechanism likely involving G2/M phase cell cycle arrest through ROS upregulation and activation of the ERK signaling pathway.
PDF
2
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
