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To explore the effect of Wuzi Yanzong pill on testicular dysfunction in subacute aging rats through metabolomics.
Forty-two rats were subcutaneously injected with 200 mg·kg-1 D-galactose (D-Gal) to induce a subacute aging model. After successful modeling, the rats were randomly divided into four groups: model group, control group, exprimental-L group, exprimental-H group, each with 9 rats. Eight normal rats without D-Gal treatment were used as the normal group. The exprimental-L,-H groups were administered Wuzi Yanzong pill at doses of 0.54 and 2.16 g·kg-1 by gavage, respectively, while the control group was given 300 mg·kg-1 metformin by gavage. The normal and model groups were treated with an equal volume of distilled water by gavage. All rats were dosed once daily for 4 weeks. During this period, the each group (except for the normal group) was continued injected 200 mg·kg-1 D-Gal injections. The levels of testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) in serum were measured using enzyme-linked immunosorbent assay (ELISA). The relative expression of proteins involved in testicular secretory function [hydroxysteroid 17-beta dehydrogenase 3 (HSD17B3), cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1), steroidogenic acute regulatory protein (STAR)] was determined by Western blot. Metabolomics analysis was performed to study metabolic changes in the testicular tissue.
The serum T levels in the normal, model, control, experimental-L, and experimental-H groups were (8.67±1.71), (1.95±0.46), (6.50±1.15), (2.45±0.49) and (5.81±0.96) pg·mL-1, respectively; the FSH levels were (28.53±4.16), (46.73±3.72), (30.18±5.17), (35.35±1.90) and (30.31±4.35) mU·mL-1, respectively; the LH levels were (22.66±3.97), (37.89±4.16), (24.28±3.75), (37.09±3.98) and (28.35±4.38) mU·mL-1, respectively; the relative protein expression levels of HSD17B3 were 1.04±0.05, 0.29±0.14, 0.78±0.18, 0.71±0.13 and 0.85±0.18; the relative protein expression levels of CYP11A1 were 1.23±0.15, 0.62±0.08, 1.01±0.05, 0.77±0.15 and 1.24±0.09; the relative protein expression levels of STAR were 0.97±0.12, 0.21±0.05, 0.79±0.19, 0.62±0.23 and 0.96±0.25, respectively. The model group showed significant differences in these indicators when compared with the control and experimental-H groups (all P<0.05). Metabolomics analysis identified six differential metabolites in the experimental-H group compared to the model group. These metabolites were mainly involved in steroid hormone biosynthesis, ovarian steroidogenesis, lipid metabolism, and biotin metabolism.
In vivo and metabolomics studies show that Wuzi Yanzong pill improves antioxidant capacity in the testes, reduces oxidative stress damage, delays aging, and effectively treats testicular dysfunction, thereby improving testicular function.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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