PDF
Full
Outline
To explore the effects of essential oil of Ligusticum chuanxiong (LCEO) on primary dysmenorrhea (PD) and preliminarily its mechanism of action.
Forty-eight female non-pregnant KM mice were randomly divided into blank group, model group, positive control group and experimental -L, -M, -H groups. Except for the blank group, the remaining groups were gavaged with estradiol valerate to establish the PD mouse model. Starting from the 7th day, the blank group and the model group were gavaged with equal volumes of distilled water in the morning, the positive control group was administered 120 mg·kg-1 of ibuprofen solution by gastric lavage, while the low, medium, and high dose experimental groups were gavaged with 22, 44 and 88 mg·kg-1 of LCEO, respectively, for 5 consecutive days. Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of oxytocin (OT) and prostaglandin F2α (PGF2α) in mice uterine. Using the oxytocin-induced rat isolated uterine contraction model, the study explored the mechanism of LCEO in relaxing uterine smooth muscle through preincubation with different blocking agents (Indomethacin, phentolamine mesylate, propranolol hydrochloride, atropine sulfate, diphenhydramine hydrochloride, ranitidine hydrochloride and nifedipine).
The OT contents in the blank group, model group, positive control group and experimental -L, -M, -H groups were (3.57±0.81), (5.54±0.60), (4.63±0.75), (4.15±0.76), (4.04±1.19) and (3.67±0.66) pg·mg-1; the PGF2α contents were (1.22±0.38), (2.53±0.45), (1.84±0.52), (1.30±0.31), (1.21±0.35) and (0.48±0.15) pg·mg-1, respectively. The above indexes in the model group were compared with the experimental -L, -M, -H and blank, positive control groups, there were statistically significant differences in all comparisons (P<0.01, P<0.05). After preincubation with indomethacin, phentolamine mesylate, and atropine sulfate, the relaxant effect on uterine smooth muscle of LCEO was significantly weakened. After preincubation with propranolol hydrochloride, diphenhydramine hydrochloride, ranitidine hydrochloride and nifedipine, the relaxant effect on uterine smooth muscle of LCEO did not show any significant changes.
LCEO has a significant effect on primary dysmenorrhea, and its mechanism may be related to inhibiting cyclooxygenase and blocking receptors α and M.
PDF
2
1
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
