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To investigate the effect of pyrotinib on ferroptosis in gastric cancer cells through the regulation of the tumor protein 53 (p53)/solute carrier family 7 member 11 (SLC7A11) axis by the long noncoding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16).
Human gastric cancer cells (NCI-N87) were selected and divided into NCI-N87 group (NCI-N87 cells were cultured normally without treatment), experimental group (NCI-N87 cells treated with 0.5 μmol·L-1 pyrotinib for 72 h), and oe-NC group (based on the transfection of oe-NC in experimental group), LncRNA SNHG16 group (based on experimental group transfected with oe-LncRNA SNHG16). Real-time fluorescence quantitative polymerase chain reaction and fluorescence in situ hybridisation were used to determine the expression level of LncRNA SNHG16, cell viability was detected by cell counting kit-8; invasive ability was detected by Transwell assay; reactive oxygen species (ROS) fluorescence level was detected by fluorescent probe staining of dihydroethidium (DHE); malondialdehyde (MDA) level and glutathione (GSH) activity were detected by kits; and the expression level of p53 and SLC7A11 protein was detected by Western blot.
The relative expression levels of LncRNA SNHG16 in the NCI-N87 group, the experimental group, the oe-NC group and the LncRNA SNHG16 group were 1.00±0.18, 0.39±0.06, 0.41±0.07 and 0.80±0.15, respectively; the relative fluorescence intensities of LncRNA SNHG16 were 1.00±0.16, 0.27±0.05, 0.23±0.04 and 0.81±0.17, respectively; the number of cell invasion was (82.16±14.67), (29.03±4.75), (31.82±5.69) and (74.98±11.34) pieces, respectively; the relative fluorescence levels of ROS were 1.00±0.15, 4.57±0.86, 3.95±0.61 and 1.54±0.33, respectively; MDA levels were (5.49±1.08), (11.27±1.93), (10.85±1.64) and (6.73±0.95) μmol·mg-1, respectively; GSH relative activities were 1.00±0.00, 0.54±0.09, 0.56±0.07 and 0.83±0.14, respectively; the relative expression levels of p53 protein were 1.00±0.17, 2.26±0.38, 1.98±0.32 and 1.24±0.19, respectively; the relative expression levels of SLC7A11 protein were 1.00±0.13, 0.34±0.05, 0.41±0.09 and 0.79±0.11, respectively. The differences in the above indicators between the NCI-N87 group and the experimental group, as well as between the oe-NC group and the LncRNA SNHG16 group, were all statistically significant (P<0.001).
Pyrotinib can regulate the p53/SLC7A11 signaling pathway by inhibiting the expression of LncRNA SNHG16 to induce ferroptosis of gastric cancer cells and inhibit the proliferation and invasion of gastric cancer cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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